Polysaccharide-based artificial extracellular matrix: Preparation and characterization of three-dimensional, macroporous chitosan, and heparin composite scaffold

Shu Huei Yu, Yu B. Wu, Fwu Long Mi, Shin Shing Shyu

研究成果: 雜誌貢獻文章

12 引文 斯高帕斯(Scopus)

摘要

Scaffold-guided tissue engineering based on synthetic and natural occurring polymers has gained many interests in recent year. In this study, the development of a chitosan-heparin artificial extracellular matrix (AECM) is reported. Three-dimensional, macroporous composite AECMs composed of heparin (Hep) and chitosan (Chito) were prepared by an interpolyelectrolyte complex/ lyophilization method. The Chito-Hep composite AECMs were, respectively, crosslinked with glutaraldehyde, as well as cocrosslinked with N,N-(3-dimethylaminopropyl)-N′-ethyl carbodiimide (EDC/NHS) and N-hydroxysuccinimide (NHS). The crosslinking reactions were examined by FT-IR analysis. In physiological buffer solution (PBS), the EDC/NHS-crosslinked Chito-Hep composite AECM showed a relative lower water retention ratio than its glutaraldehyde-crosslinked counterparts. The EDC/NHS-crosslinked Chito-Hep composite AECMs showed excellent biocompatibility, according to the results of the in vitro cytotoxic test. This result suggested that the EDC/NHS-crosslinked Chito-Hep composite AECMs might be a potential biomaterial for scaffold-guided tissue engineering applications.

原文英語
頁(從 - 到)3639-3644
頁數6
期刊Journal of Applied Polymer Science
109
發行號6
DOIs
出版狀態已發佈 - 九月 15 2008
對外發佈Yes

ASJC Scopus subject areas

  • Chemistry(all)
  • Materials Chemistry
  • Polymers and Plastics
  • Surfaces, Coatings and Films

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