Polymorphisms of human 8-oxoguanine DNA glycosylase 1 and 8-hydroxydeoxyguanosine increase susceptibility to arsenic methylation capacity-related urothelial carcinoma

Chao Yuan Huang, Yeong Shiau Pu, Horng Sheng Shiue, Wei Jen Chen, Ying-Chin Lin, Yu Mei Hsueh

研究成果: 雜誌貢獻文章

4 引文 (Scopus)

摘要

Arsenic causes oxidative stress in cultured animal and human cells, and it is a well-documented human carcinogen. We conducted a hospital-based case–control study including 167 cases of urothelial carcinoma (UC) and 334 age- and gender-matched healthy controls to evaluate the relationships between urinary arsenic profiles, urinary 8-hydroxydeoxyguanosine (8-OHdG) levels, and human 8-oxoguanine DNA glycosylase (hOGG1) genotypes and UC. The urinary arsenic species were analyzed by high-performance liquid chromatography and hydride generator-atomic absorption spectrometry. Genotyping for hOGG1 (Ser326Cys) and hOGG1 (−15C>G) was performed using the Sequenom MassARRAY platform with iPLEX Gold chemistry. Urinary 8-OHdG was measured with high-sensitivity enzyme-linked immunosorbent assay kits. The results indicated that the hOGG1 326 Cys/Cys genotype and the hOGG1 −15C>G G/G genotype were associated with an increased risk of UC (OR [95 % CI] 1.57 [1.04–2.35] and 1.57 [1.04–2.35], respectively). Participants with high urinary total arsenic, regardless of the haplotype of hOGG1 Ser326Cys and the −15C>G polymorphism, had significantly higher urinary 8-OHdG compared to participants with low urinary total arsenic. This is the first study to investigate the joint effects of high urinary total arsenic or inefficient arsenic methylation capacity indices, and the high-risk G–G haplotype of hOGG1 on the risk of UC. The findings are especially meaningful for participants with risk factors such as high urinary total arsenic, inefficient arsenic methylation indices, high urinary 8-OHdG, and the high-risk G–G haplotype of hOGG1 which are all associated with an increased UC risk.
原文英語
期刊Archives of Toxicology
DOIs
出版狀態接受/付印 - 九月 10 2015

指紋

Methylation
Arsenic
Polymorphism
Carcinoma
Haplotypes
Genotype
human oxoguanine glycosylase 1
8-oxo-7-hydrodeoxyguanosine
Immunosorbents
Atomic absorption spectrometry
Oxidative stress
High performance liquid chromatography
Hydrides
Gold
Carcinogens
Assays
Spectrum Analysis
Animals
Oxidative Stress
Enzyme-Linked Immunosorbent Assay

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

引用此文

@article{0656197c0655473482e781549d5fa697,
title = "Polymorphisms of human 8-oxoguanine DNA glycosylase 1 and 8-hydroxydeoxyguanosine increase susceptibility to arsenic methylation capacity-related urothelial carcinoma",
abstract = "Arsenic causes oxidative stress in cultured animal and human cells, and it is a well-documented human carcinogen. We conducted a hospital-based case–control study including 167 cases of urothelial carcinoma (UC) and 334 age- and gender-matched healthy controls to evaluate the relationships between urinary arsenic profiles, urinary 8-hydroxydeoxyguanosine (8-OHdG) levels, and human 8-oxoguanine DNA glycosylase (hOGG1) genotypes and UC. The urinary arsenic species were analyzed by high-performance liquid chromatography and hydride generator-atomic absorption spectrometry. Genotyping for hOGG1 (Ser326Cys) and hOGG1 (−15C>G) was performed using the Sequenom MassARRAY platform with iPLEX Gold chemistry. Urinary 8-OHdG was measured with high-sensitivity enzyme-linked immunosorbent assay kits. The results indicated that the hOGG1 326 Cys/Cys genotype and the hOGG1 −15C>G G/G genotype were associated with an increased risk of UC (OR [95 {\%} CI] 1.57 [1.04–2.35] and 1.57 [1.04–2.35], respectively). Participants with high urinary total arsenic, regardless of the haplotype of hOGG1 Ser326Cys and the −15C>G polymorphism, had significantly higher urinary 8-OHdG compared to participants with low urinary total arsenic. This is the first study to investigate the joint effects of high urinary total arsenic or inefficient arsenic methylation capacity indices, and the high-risk G–G haplotype of hOGG1 on the risk of UC. The findings are especially meaningful for participants with risk factors such as high urinary total arsenic, inefficient arsenic methylation indices, high urinary 8-OHdG, and the high-risk G–G haplotype of hOGG1 which are all associated with an increased UC risk.",
keywords = "8-Hydroxydeoxyguanosine, 8-Oxoguanine DNA glycosylase, Arsenic, Haplotype, Methylation, Urothelial carcinoma",
author = "Huang, {Chao Yuan} and Pu, {Yeong Shiau} and Shiue, {Horng Sheng} and Chen, {Wei Jen} and Ying-Chin Lin and Hsueh, {Yu Mei}",
year = "2015",
month = "9",
day = "10",
doi = "10.1007/s00204-015-1590-x",
language = "English",
journal = "Archiv fur Toxikologie",
issn = "0003-9446",
publisher = "Springer Verlag",

}

TY - JOUR

T1 - Polymorphisms of human 8-oxoguanine DNA glycosylase 1 and 8-hydroxydeoxyguanosine increase susceptibility to arsenic methylation capacity-related urothelial carcinoma

AU - Huang, Chao Yuan

AU - Pu, Yeong Shiau

AU - Shiue, Horng Sheng

AU - Chen, Wei Jen

AU - Lin, Ying-Chin

AU - Hsueh, Yu Mei

PY - 2015/9/10

Y1 - 2015/9/10

N2 - Arsenic causes oxidative stress in cultured animal and human cells, and it is a well-documented human carcinogen. We conducted a hospital-based case–control study including 167 cases of urothelial carcinoma (UC) and 334 age- and gender-matched healthy controls to evaluate the relationships between urinary arsenic profiles, urinary 8-hydroxydeoxyguanosine (8-OHdG) levels, and human 8-oxoguanine DNA glycosylase (hOGG1) genotypes and UC. The urinary arsenic species were analyzed by high-performance liquid chromatography and hydride generator-atomic absorption spectrometry. Genotyping for hOGG1 (Ser326Cys) and hOGG1 (−15C>G) was performed using the Sequenom MassARRAY platform with iPLEX Gold chemistry. Urinary 8-OHdG was measured with high-sensitivity enzyme-linked immunosorbent assay kits. The results indicated that the hOGG1 326 Cys/Cys genotype and the hOGG1 −15C>G G/G genotype were associated with an increased risk of UC (OR [95 % CI] 1.57 [1.04–2.35] and 1.57 [1.04–2.35], respectively). Participants with high urinary total arsenic, regardless of the haplotype of hOGG1 Ser326Cys and the −15C>G polymorphism, had significantly higher urinary 8-OHdG compared to participants with low urinary total arsenic. This is the first study to investigate the joint effects of high urinary total arsenic or inefficient arsenic methylation capacity indices, and the high-risk G–G haplotype of hOGG1 on the risk of UC. The findings are especially meaningful for participants with risk factors such as high urinary total arsenic, inefficient arsenic methylation indices, high urinary 8-OHdG, and the high-risk G–G haplotype of hOGG1 which are all associated with an increased UC risk.

AB - Arsenic causes oxidative stress in cultured animal and human cells, and it is a well-documented human carcinogen. We conducted a hospital-based case–control study including 167 cases of urothelial carcinoma (UC) and 334 age- and gender-matched healthy controls to evaluate the relationships between urinary arsenic profiles, urinary 8-hydroxydeoxyguanosine (8-OHdG) levels, and human 8-oxoguanine DNA glycosylase (hOGG1) genotypes and UC. The urinary arsenic species were analyzed by high-performance liquid chromatography and hydride generator-atomic absorption spectrometry. Genotyping for hOGG1 (Ser326Cys) and hOGG1 (−15C>G) was performed using the Sequenom MassARRAY platform with iPLEX Gold chemistry. Urinary 8-OHdG was measured with high-sensitivity enzyme-linked immunosorbent assay kits. The results indicated that the hOGG1 326 Cys/Cys genotype and the hOGG1 −15C>G G/G genotype were associated with an increased risk of UC (OR [95 % CI] 1.57 [1.04–2.35] and 1.57 [1.04–2.35], respectively). Participants with high urinary total arsenic, regardless of the haplotype of hOGG1 Ser326Cys and the −15C>G polymorphism, had significantly higher urinary 8-OHdG compared to participants with low urinary total arsenic. This is the first study to investigate the joint effects of high urinary total arsenic or inefficient arsenic methylation capacity indices, and the high-risk G–G haplotype of hOGG1 on the risk of UC. The findings are especially meaningful for participants with risk factors such as high urinary total arsenic, inefficient arsenic methylation indices, high urinary 8-OHdG, and the high-risk G–G haplotype of hOGG1 which are all associated with an increased UC risk.

KW - 8-Hydroxydeoxyguanosine

KW - 8-Oxoguanine DNA glycosylase

KW - Arsenic

KW - Haplotype

KW - Methylation

KW - Urothelial carcinoma

UR - http://www.scopus.com/inward/record.url?scp=84941345270&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84941345270&partnerID=8YFLogxK

U2 - 10.1007/s00204-015-1590-x

DO - 10.1007/s00204-015-1590-x

M3 - Article

JO - Archiv fur Toxikologie

JF - Archiv fur Toxikologie

SN - 0003-9446

ER -