One-dimensional thin-layer chromatography on polyamide plates offers an easy and rapid identification of O-phosphotyrosine. The thin-layer plate is developed for 30 min in 5% propionic acid containing 0.013%-0.025% sodium dodecyl sulfate. O-Phosphotyrosine, with Rf = 0.54, can be well separated from O-phosphothreonine and O-phosphoserine, which comigrate at Rf = 0.72.
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology
Chang, W. C., Lee, M. L., Chou, C. K., & Lee, S. C. (1983). Polyamide thin-layer chromatography of phosphorylated tyrosine, threonine, and serine. Analytical Biochemistry, 132(2), 342-344. https://doi.org/10.1016/0003-2697(83)90018-0