Polyamide thin-layer chromatography of phosphorylated tyrosine, threonine, and serine

Wen Chang Chang, Muh Lin Lee, Chen Kung Chou, Sheng Chung Lee

研究成果: 雜誌貢獻文章

9 引文 斯高帕斯(Scopus)

摘要

One-dimensional thin-layer chromatography on polyamide plates offers an easy and rapid identification of O-phosphotyrosine. The thin-layer plate is developed for 30 min in 5% propionic acid containing 0.013%-0.025% sodium dodecyl sulfate. O-Phosphotyrosine, with Rf = 0.54, can be well separated from O-phosphothreonine and O-phosphoserine, which comigrate at Rf = 0.72.
原文英語
頁(從 - 到)342-344
頁數3
期刊Analytical Biochemistry
132
發行號2
DOIs
出版狀態已發佈 - 七月 15 1983
對外發佈Yes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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