Pluripotency of mouse spermatogonial stem cells maintained by IGF-1- Dependent pathway

Yen-Hua Huang, Cheng Chieh Chin, Ng Nerng Ho, Chuan Kai Chou, Chia Ning Shen, Hung Chih Kuo, Tsai Jung Wu, Yu Chih Wu, Yu Ching Hung, Chih Cheng Chang, Thai Y. Ling

研究成果: 雜誌貢獻文章

82 引文 斯高帕斯(Scopus)

摘要

Recent studies indicate that neonatal spermatogonial stem cells (SSCs) possess pluripotency. However, the mechanisms that regulate the pluripotent differentiation capacity of SSCs remain unclear. Here, we describe a new method to clonally derive pluripotent SSCs from neonatal mouse testis. By coculturing with testicular stromal cells, SSCs can be maintained and expanded in serum-free conditions. Unlike endogenous SSCs, these in vitro expanded SSCs showed strong alkaline phosphatase (AP) activity and displayed characteristics of embryonic stem cells and primordial germ cells, which were therefore designated as AP + germline stem cells (AP+GSCs). The pluripotency of AP+GSCs was confirmed by in vitro differentiation toward hepatic and neuronal lineages and formation of embryonic chimeras after injection into blastocysts. Further investigation revealed that insulin-like growth factor-1 (IGF-1) secreted from Leydig cells was a key factor involved in maintaining the pluripotency of AP+GSCs. The blockage of IGF-1 receptor phosphorylation and its downstream PI3K pathway by PPP or LY294002 dramatically reduced their AP activity and expression of pluripotent genes, such as Oct-4, Blimp1, and Nanog. In conclusion, the present study demonstrated that IGF-1 secreted by testicular Leydig cells plays an important role in maintaining the pluripotency of SSCs in culture, which provides an insight into the molecular mechanism underlying germ cell pluripotency.
原文英語
頁(從 - 到)2076-2087
頁數12
期刊FASEB Journal
23
發行號7
DOIs
出版狀態已發佈 - 七月 2009

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Genetics
  • Molecular Biology
  • Medicine(all)

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    Huang, Y-H., Chin, C. C., Ho, N. N., Chou, C. K., Shen, C. N., Kuo, H. C., Wu, T. J., Wu, Y. C., Hung, Y. C., Chang, C. C., & Ling, T. Y. (2009). Pluripotency of mouse spermatogonial stem cells maintained by IGF-1- Dependent pathway. FASEB Journal, 23(7), 2076-2087. https://doi.org/10.1096/fj.08-121939