Platonin preserves blood-brain barrier integrity in septic rats

Chia Tse Yeh, Ming Chang Kao, Cay Huyen Chen, Chun Jen Huang

研究成果: 雜誌貢獻文章

9 引文 斯高帕斯(Scopus)

摘要

Objectives Platonin possesses potent anti-inflammatory and antioxidative capacities. Because systemic inflammation and oxidative stress are crucial in mediating sepsis-induced blood-brain barrier (BBB) integrity loss, this study elucidated the effects of platonin on preserving BBB integrity in septic rats. Methods A total of 72 adult male rats (200-250 g) were randomized to receive cecal ligation and puncture (CLP), CLP plus platonin, sham operation, or sham operation plus platonin (n = 18 in each group). Systemic inflammation and oxidation levels and BBB integrity in the surviving rats were determined after 24-hour monitoring. Results Plasma levels of interleukin-6 (IL-6) and malondialdehyde (MDA) - markers of systemic inflammation and oxidation - and the grading of Evans blue staining of the brains, BBB permeability to Evans blue dye, and brain edema levels - markers of BBB integrity - in rats that received CLP were significantly higher than rats that received sham operation (all p < 0.001). By contrast, the plasma levels of IL-6 (p < 0.001) and MDA (p < 0.001), and the grading of Evans blue staining (p = 0.015), BBB permeability to Evans blue dye (p = 0.043), and brain edema levels (p = 0.034) in rats that received CLP plus platonin were significantly lower than rats that received CLP. Experimental data further revealed that the concentration of tight junction protein claudin-5, a major structural component of BBB, in rats that received CLP was significantly lower than rats that received CLP plus platonin (p = 0.023). Conclusion Platonin could attenuate sepsis-induced BBB integrity loss in rats.
原文英語
頁(從 - 到)12-15
頁數4
期刊Acta Anaesthesiologica Taiwanica
53
發行號1
DOIs
出版狀態已發佈 - 三月 1 2015
對外發佈Yes

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine

指紋 深入研究「Platonin preserves blood-brain barrier integrity in septic rats」主題。共同形成了獨特的指紋。

  • 引用此