Platelets stimulate aortic smooth muscle cell migration in vitro. Involvement of 12-l-hydroxy-5,8,10,14-eicosatetraenoic acid

Junko Nakao, Toshiro Ooyama, Wen Chang Chang, Sei itsu Murota, Hajime Orimo

研究成果: 雜誌貢獻文章

68 引文 (Scopus)

摘要

The migration of rat aortic smooth muscle cells was measured in modified Boyden chambers. Smooth muscle cells were motile in vitro and their migration was stimulated (time- and dose-dependently) by a platelet-derived factor. Treatment of platelets with indomethacin resulted in a significant increase in smooth muscle cell migration, whereas treatment with 5,8,11,14-eicosatetraenoic acid inhibited it. Purified 12-l-hydroxy-5,8,10,14-eicosatetraenoic acid at a very low concentration (6 × 10-15-6 ×-13 g/ml) significantly stimulated smooth muscle cell migration. The locomotion induced by 12-l-hydroxy-5,8,10,14-eicosatetraenoic acid was chemokinetic. These findings point to the physiological importance of a platelet 12-lipoxygenase product of arachidonic acid in the early phase of atherosclerosis.

原文英語
頁(從 - 到)143-150
頁數8
期刊Atherosclerosis
43
發行號2-3
DOIs
出版狀態已發佈 - 1982
對外發佈Yes

指紋

12-Hydroxy-5,8,10,14-eicosatetraenoic Acid
Smooth Muscle Myocytes
Cell Movement
Blood Platelets
Arachidonate 12-Lipoxygenase
Arachidonic Acids
Locomotion
Indomethacin
Atherosclerosis
In Vitro Techniques

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

引用此文

Platelets stimulate aortic smooth muscle cell migration in vitro. Involvement of 12-l-hydroxy-5,8,10,14-eicosatetraenoic acid. / Nakao, Junko; Ooyama, Toshiro; Chang, Wen Chang; Murota, Sei itsu; Orimo, Hajime.

於: Atherosclerosis, 卷 43, 編號 2-3, 1982, p. 143-150.

研究成果: 雜誌貢獻文章

Nakao, Junko ; Ooyama, Toshiro ; Chang, Wen Chang ; Murota, Sei itsu ; Orimo, Hajime. / Platelets stimulate aortic smooth muscle cell migration in vitro. Involvement of 12-l-hydroxy-5,8,10,14-eicosatetraenoic acid. 於: Atherosclerosis. 1982 ; 卷 43, 編號 2-3. 頁 143-150.
@article{d5d32d05b9b54f669be29ec235b8df81,
title = "Platelets stimulate aortic smooth muscle cell migration in vitro. Involvement of 12-l-hydroxy-5,8,10,14-eicosatetraenoic acid",
abstract = "The migration of rat aortic smooth muscle cells was measured in modified Boyden chambers. Smooth muscle cells were motile in vitro and their migration was stimulated (time- and dose-dependently) by a platelet-derived factor. Treatment of platelets with indomethacin resulted in a significant increase in smooth muscle cell migration, whereas treatment with 5,8,11,14-eicosatetraenoic acid inhibited it. Purified 12-l-hydroxy-5,8,10,14-eicosatetraenoic acid at a very low concentration (6 × 10-15-6 ×-13 g/ml) significantly stimulated smooth muscle cell migration. The locomotion induced by 12-l-hydroxy-5,8,10,14-eicosatetraenoic acid was chemokinetic. These findings point to the physiological importance of a platelet 12-lipoxygenase product of arachidonic acid in the early phase of atherosclerosis.",
keywords = "12-l-Hydroxy-5,8,10,14-eicosatetraenoic acid, Atherosclerosis, Chemokinesis, Platelet, Smooth muscle cell migration",
author = "Junko Nakao and Toshiro Ooyama and Chang, {Wen Chang} and Murota, {Sei itsu} and Hajime Orimo",
year = "1982",
doi = "10.1016/0021-9150(82)90018-1",
language = "English",
volume = "43",
pages = "143--150",
journal = "Atherosclerosis",
issn = "0021-9150",
publisher = "Elsevier Ireland Ltd",
number = "2-3",

}

TY - JOUR

T1 - Platelets stimulate aortic smooth muscle cell migration in vitro. Involvement of 12-l-hydroxy-5,8,10,14-eicosatetraenoic acid

AU - Nakao, Junko

AU - Ooyama, Toshiro

AU - Chang, Wen Chang

AU - Murota, Sei itsu

AU - Orimo, Hajime

PY - 1982

Y1 - 1982

N2 - The migration of rat aortic smooth muscle cells was measured in modified Boyden chambers. Smooth muscle cells were motile in vitro and their migration was stimulated (time- and dose-dependently) by a platelet-derived factor. Treatment of platelets with indomethacin resulted in a significant increase in smooth muscle cell migration, whereas treatment with 5,8,11,14-eicosatetraenoic acid inhibited it. Purified 12-l-hydroxy-5,8,10,14-eicosatetraenoic acid at a very low concentration (6 × 10-15-6 ×-13 g/ml) significantly stimulated smooth muscle cell migration. The locomotion induced by 12-l-hydroxy-5,8,10,14-eicosatetraenoic acid was chemokinetic. These findings point to the physiological importance of a platelet 12-lipoxygenase product of arachidonic acid in the early phase of atherosclerosis.

AB - The migration of rat aortic smooth muscle cells was measured in modified Boyden chambers. Smooth muscle cells were motile in vitro and their migration was stimulated (time- and dose-dependently) by a platelet-derived factor. Treatment of platelets with indomethacin resulted in a significant increase in smooth muscle cell migration, whereas treatment with 5,8,11,14-eicosatetraenoic acid inhibited it. Purified 12-l-hydroxy-5,8,10,14-eicosatetraenoic acid at a very low concentration (6 × 10-15-6 ×-13 g/ml) significantly stimulated smooth muscle cell migration. The locomotion induced by 12-l-hydroxy-5,8,10,14-eicosatetraenoic acid was chemokinetic. These findings point to the physiological importance of a platelet 12-lipoxygenase product of arachidonic acid in the early phase of atherosclerosis.

KW - 12-l-Hydroxy-5,8,10,14-eicosatetraenoic acid

KW - Atherosclerosis

KW - Chemokinesis

KW - Platelet

KW - Smooth muscle cell migration

UR - http://www.scopus.com/inward/record.url?scp=0019946806&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019946806&partnerID=8YFLogxK

U2 - 10.1016/0021-9150(82)90018-1

DO - 10.1016/0021-9150(82)90018-1

M3 - Article

C2 - 7115458

AN - SCOPUS:0019946806

VL - 43

SP - 143

EP - 150

JO - Atherosclerosis

JF - Atherosclerosis

SN - 0021-9150

IS - 2-3

ER -