Plasmin-induced migration of endothelial cells: A potential target for the anti-angiogenic action of angiostatin

Takehiko Tarui, Mousumi Majumdar, Lindsey A. Miles, Wolfram Ruf, Yoshikazu Takada

研究成果: 雜誌貢獻文章

106 引文 斯高帕斯(Scopus)

摘要

Angiostatin, a plasminogen fragment containing 3-4 N-terminal kringle domains, is a potent inhibitor of tumor-induced angiogenesis, but its mechanism of action is unclear. Angiostatin is a ligand for integrin αvβ3 but does not induce stress fiber formation upon integrin binding, suggesting that angiostatin is a potential integrin antagonist. Plasmin, the parent molecule of angiostatin and a major extracellular protease, induces platelet aggregation, migration of peripheral blood monocytes, and release of arachidonate and leukotriene from several cell types. In the current study, we found that plasmin specifically bound to αvβ3 through the kringle domains and induced migration of endothelial cells. In contrast, angiostatin did not induce cell migration. Notably, angiostatin, anti-αvβ3 antibodies, RGD- peptide, and a serine protease inhibitor effectively blocked plasmin-induced cell migration. These results suggest that plasmin-induced migration of endothelial cells requires αvβ3 and the catalytic activity of plasmin and that this process is a potential target for the inhibitory activity of angiostatin.

原文英語
頁(從 - 到)33564-33570
頁數7
期刊Journal of Biological Chemistry
277
發行號37
DOIs
出版狀態已發佈 - 九月 13 2002
對外發佈Yes

ASJC Scopus subject areas

  • Biochemistry

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