Plasmid linking number change induced by topoisomerase I-mediated DNA damage

Pu Duann, Mei Sun, Chin Tai Lin, Hui Zhang, Leroy-Fong Liu

研究成果: 雜誌貢獻文章同行評審

11 引文 斯高帕斯(Scopus)


The state of cellular chromatin in response to DNA damage has been examined by monitoring the change in the linking number of circular episomes. COS cells transfected with an SV40-based vector were treated with camptothecin (CPT), a eukaryotic DNA topoisomerase I (TOP1) poison which induces TOP1-mediated DNA damage. Within minutes, a large increase in the linking number (over 10 linking number) of a small fraction (5-15%) of the episomal DNA was observed. A similar CPT-induced increase in plasmid DNA linking number was observed in Saccharomyces cerevisae expressing human DNA TOP1. In this case, the majority of the plasmid DNA can undergo rapid relaxation. The large increase in the plasmid linking number suggests major chromatin structural reorganization in response to TOP I-mediated DNA damage.

頁(從 - 到)2905-2911
期刊Nucleic Acids Research
出版狀態已發佈 - 七月 15 1999

ASJC Scopus subject areas

  • Genetics

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