Placenta-derived multipotent cells exhibit immunosuppressive properties that are enhanced in the presence of interferon-γ

Chun Jung Chang, Men Luh Yen, Yao Chang Chen, Chih Cheng Chien, Hsing I. Huang, Chyi Huey Bai, B. Linju Yen

研究成果: 雜誌貢獻文章同行評審

214 引文 斯高帕斯(Scopus)

摘要

Several types of nonhematopoietic stem cells, including bone marrow mesenchymal stem cells (BMMSCs) and embryonic stem cells, have been shown to have immunosuppressive properties. We show that human placenta-derived multipotent cells (PDMCs), which are isolated from a source without ethical concern and harbor multilineage differentiation potential, have strong immunosuppressive properties. PDMCs suppress both mitogen-induced and allogeneic lymphocyte proliferation in both CD4 and CD8 populations. The immunosuppression seen with PDMCs was significantly stronger than that with BMMSCs. Both PDMCs and BMMSCs express indoleamine 2,3-dioxygenase, but only PDMCs are positive for intracellular human leukocyte antigen-G (HLA). Mechanistically, suppression of lymphocyte reactivity by PDMCs is not due to cell death but to decreased cell proliferation and increased numbers of regulatory T cells. Addition of neutralizing antibodies to interleukin-10 and transforming growth factor (TGF)-β partially restored lymphocyte proliferation. Unlike BMMSCs, PDMCs treated with interferon-γ for 3 days only very minimally upregulated HLA-DR. On the contrary, PD-L1, a cell surface marker that plays an inhibitory role in T-cell activation, was upregulated and TGF-β expression was seen. The immunosuppressive properties of PDMCs, along with their multilineage differentiation potential, ease of accessibility, and abundant cell numbers, may render these cells as good potential sources for future therapeutic applications.

原文英語
頁(從 - 到)2466-2477
頁數12
期刊Stem Cells
24
發行號11
DOIs
出版狀態已發佈 - 2006
對外發佈Yes

ASJC Scopus subject areas

  • Cell Biology

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