Physiological and functional interactions between Tcf4 and Daxx in colon cancer cells

Shu Ling Tzeng, Yu Wen Cheng, Ching Hao Li, Young Sun Lin, Hey Chi Hsu, Jaw Jou Kang

研究成果: 雜誌貢獻文章同行評審

28 引文 斯高帕斯(Scopus)


Daxx, a human cell death-associated protein, was isolated as a Tcf4-interacting protein, using a yeast two-hybrid screen. Co- immunoprecipitation in HEK-293T cells and yeast two-hybrid screen in Y190 cells were performed to identify the interaction between Tcf4 with Daxx and to map the binding regions of Tcf4. In the nucleus, Daxx reduced DNA binding activity of Tcf4 and repressed Tcf4 transcriptional activity. Overexpression of Daxx altered the expression of genes downstream of Tcf4, including cyclin D1 and Hath-1, and induced G1 phase arrest in colon cancer cells. A reduction in Daxx protein expression was also observed in colon adenocarcinoma tissue when compared with normal colon tissue. This evidence suggests a possible physiological function of Daxx, via interaction with Tcf4, to regulate proliferation and differentiation of colon cells.
頁(從 - 到)15405-15411
期刊Journal of Biological Chemistry
出版狀態已發佈 - 6月 2 2006

ASJC Scopus subject areas

  • 生物化學
  • 分子生物學
  • 細胞生物學


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