Physicochemical and biological characterization of single-walled and double-walled carbon nanotubes in biological media

Wen Te Liu, Mauo Ying Bien, Kai Jen Chuang, Ta Yuan Chang, Tim Jones, Kelly BéruBé, Georgi Lalev, Dai Hua Tsai, Hsiao Chi Chuang, Tsun Jen Cheng

研究成果: 雜誌貢獻文章同行評審

12 引文 斯高帕斯(Scopus)

摘要

To study the toxicity of nanoparticles under relevant conditions, it is important to reproducibly disperse nanoparticles in biological media in in vitro and in vivo studies. Here, single-walled nanotubes (SWNTs) and double-walled nanotubes (DWNTs) were physicochemically and biologically characterized when dispersed in phosphate-buffered saline (PBS) and bovine serum albumin (BSA). BSA-SWNT/DWNT interaction resulted in a reduction of aggregation and an increase in particle stabilization. Based on the protein sequence coverage and protein binding results, DWNTs exhibited higher protein binding than SWNTs. SWNT and DWNT suspensions in the presence of BSA increased interleukin-6 (IL-6) levels and reduced tumor necrosis factor-alpha (TNF-α) levels in A549 cells as compared to corresponding samples in the absence of BSA. We next determined the effects of SWNTs and DWNTs on pulmonary protein modification using bronchoalveolar lavage fluid (BALF) as a surrogate collected form BALB/c mice. The BALF proteins bound to SWNTs (13 proteins) and DWNTs (11 proteins), suggesting that these proteins were associated with blood coagulation pathways. Lastly, we demonstrated the importance of physicochemical and biological alterations of SWNTs and DWNTs when dispersed in biological media, since protein binding may result in the misinterpretation of in vitro results and the activation of protein-regulated biological responses.
原文英語
頁(從 - 到)216-225
頁數10
期刊Journal of Hazardous Materials
280
DOIs
出版狀態已發佈 - 九月 15 2014

ASJC Scopus subject areas

  • 健康、毒理學和誘變
  • 污染
  • 廢物管理和處置
  • 環境化學
  • 環境工程
  • 醫藥 (全部)

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