Phosphorylation of Ubc9 by Cdk1 enhances SUMOylation activity

Yee Fun Su, Tsunghan Yang, Hoting Huang, Leroy-Fong Liu, Jaulang Hwang

研究成果: 雜誌貢獻文章

24 引文 (Scopus)

摘要

Increasing evidence has pointed to an important role of SUMOylation in cell cycle regulation, especially for M phase. In the current studies, we have obtained evidence through in vitro studies that the master M phase regulator CDK1/cyclin B kinase phosphorylates the SUMOylation machinery component Ubc9, leading to its enhanced SUMOylation activity. First, we show that CDK1/cyclin B, but not many other cell cycle kinases such as CDK2/cyclin E, ERK1, ERK2, PKA and JNK2/SAPK1, specifically enhances SUMOylation activity. Second, CDK1/cyclin B phosphorylates the SUMOylation machinery component Ubc9, but not SAE1/SAE2 or SUMO1. Third, CDK1/cyclin B-phosphorylated Ubc9 exhibits increased SUMOylation activity and elevated accumulation of the Ubc9-SUMO1 thioester conjugate. Fourth, CDK1/cyclin B enhances SUMOylation activity through phosphorylation of Ubc9 at serine 71. These studies demonstrate for the first time that the cell cycle-specific kinase CDK1/cyclin B phosphorylates a SUMOylation machinery component to increase its overall SUMOylation activity, suggesting that SUMOylation is part of the cell cycle program orchestrated by CDK1 through Ubc9.

原文英語
文章編號e34250
期刊PLoS One
7
發行號4
DOIs
出版狀態已發佈 - 四月 3 2012
對外發佈Yes

指紋

Sumoylation
Cyclin B
Phosphorylation
cyclins
phosphorylation
Cells
cell cycle
Machinery
Phosphotransferases
phosphotransferases (kinases)
Cell Cycle
mitosis
Cyclin E
Cell Division
Serine
in vitro studies
serine

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

引用此文

Phosphorylation of Ubc9 by Cdk1 enhances SUMOylation activity. / Su, Yee Fun; Yang, Tsunghan; Huang, Hoting; Liu, Leroy-Fong; Hwang, Jaulang.

於: PLoS One, 卷 7, 編號 4, e34250, 03.04.2012.

研究成果: 雜誌貢獻文章

Su, Yee Fun ; Yang, Tsunghan ; Huang, Hoting ; Liu, Leroy-Fong ; Hwang, Jaulang. / Phosphorylation of Ubc9 by Cdk1 enhances SUMOylation activity. 於: PLoS One. 2012 ; 卷 7, 編號 4.
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