Phosphorylation of the zebrafish M6Ab at serine 263 contributes to filopodium formation in pc12 cells and neurite outgrowth in zebrafish embryos

Kai Yun Huang, Gen Der Chen, Chia Hsiung Cheng, Kuan Ya Liao, Chin Chun Hung, Geen Dong Chang, Pung Pung Hwang, Shu Yu Lin, Ming Chieh Tsai, Kay Hooi Khoo, Ming Ting Lee, Chang Jen Huang

研究成果: 雜誌貢獻文章同行評審

15 引文 斯高帕斯(Scopus)

摘要

Background: Mammalian M6A, a member of the proteolipid protein (PLP/DM20) family expressed in neurons, was first isolated by expression cloning with a monoclonal antibody. Overexpression of M6A was shown to induce filopodium formation in neuronal cells; however, the underlying mechanism of is largely unknown. Possibly due to gene duplication, there are two M6A paralogs, M6Aa and M6Ab, in the zebrafish genome. In the present study, we used the zebrafish as a model system to investigate the role of zebrafish M6Ab in filopodium formation in PC12 cells and neurite outgrowth in zebrafish embryos. Methodology/Principal Findings: We demonstrated that zebrafish M6Ab promoted extensive filopodium formation in NGF-treated PC12 cells, which is similar to the function of mammalian M6A. Phosphorylation at serine 263 of zebrafish M6Ab contributed to this induction. Transfection of the S263A mutant protein greatly reduced filopodium formation in PC12 cells. In zebrafish embryos, only S263D could induce neurite outgrowth. Conclusions/Significance: Our results reveal that the phosphorylation status of zebrafish M6Ab at serine 263 is critical for its role in regulating filopodium formation and neurite outgrowth.

原文英語
文章編號e26461
期刊PLoS One
6
發行號10
DOIs
出版狀態已發佈 - 2011
對外發佈

ASJC Scopus subject areas

  • 農業與生物科學 (全部)
  • 生物化學、遺傳與分子生物學 (全部)
  • 醫藥 (全部)

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