Phospholipase Cζ, the trigger egg activation in mammals, is present in a non-mammalian species

Kevin Coward, C. P. Ponting, H. Y. Chang, O. Hibbitt, P. Savolainen, K. T. Jones, J. Parrington

研究成果: 雜誌貢獻文章

70 引文 (Scopus)

摘要

The activation of the egg to begin development into an embryo is triggered by a sperm-induced increase in intracellular egg Ca2+. There has been much controversy about how the sperm induces this fundamental developmental event, but recent studies suggest that, in mammals, egg activation is triggered by a testis-specific phospholipase C: PLCζ. Since the discovery of PLCζ, it has been unclear whether its role in triggering egg activation is common to all vertebrates, or is confined to mammals. Here, we demonstrate for the first time that PLCζ is present in a non-mammalian vertebrate. Using genomic and cDNA databases, we have identified the cDNA encoding a PLCζ orthologue in the domestic chicken that, like the mammalian isoforms, is a testis-specific gene. The chicken PLCζ cDNA is 2152bp in size and encodes an open reading frame of 639 amino acids. When injected into mouse oocytes, chicken PLCζ cRNA triggers Ca2+ oscillations, indicating that it has functional properties similar to those of mammalian PLCζ. Our findings suggest that PLCζ may have a universal role in triggering egg activation in vertebrates.

原文英語
頁(從 - 到)157-163
頁數7
期刊Reproduction
130
發行號2
DOIs
出版狀態已發佈 - 八月 2005
對外發佈Yes

指紋

Type C Phospholipases
Ovum
Mammals
Vertebrates
Chickens
Complementary DNA
Spermatozoa
Testis
Complementary RNA
Open Reading Frames
Oocytes
Embryonic Development
Protein Isoforms
Databases
Amino Acids
Genes

ASJC Scopus subject areas

  • Obstetrics and Gynaecology
  • Cell Biology
  • Endocrinology
  • Embryology

引用此文

Coward, K., Ponting, C. P., Chang, H. Y., Hibbitt, O., Savolainen, P., Jones, K. T., & Parrington, J. (2005). Phospholipase Cζ, the trigger egg activation in mammals, is present in a non-mammalian species. Reproduction, 130(2), 157-163. https://doi.org/10.1530/rep.1.00707

Phospholipase Cζ, the trigger egg activation in mammals, is present in a non-mammalian species. / Coward, Kevin; Ponting, C. P.; Chang, H. Y.; Hibbitt, O.; Savolainen, P.; Jones, K. T.; Parrington, J.

於: Reproduction, 卷 130, 編號 2, 08.2005, p. 157-163.

研究成果: 雜誌貢獻文章

Coward, K, Ponting, CP, Chang, HY, Hibbitt, O, Savolainen, P, Jones, KT & Parrington, J 2005, 'Phospholipase Cζ, the trigger egg activation in mammals, is present in a non-mammalian species', Reproduction, 卷 130, 編號 2, 頁 157-163. https://doi.org/10.1530/rep.1.00707
Coward K, Ponting CP, Chang HY, Hibbitt O, Savolainen P, Jones KT 等. Phospholipase Cζ, the trigger egg activation in mammals, is present in a non-mammalian species. Reproduction. 2005 8月;130(2):157-163. https://doi.org/10.1530/rep.1.00707
Coward, Kevin ; Ponting, C. P. ; Chang, H. Y. ; Hibbitt, O. ; Savolainen, P. ; Jones, K. T. ; Parrington, J. / Phospholipase Cζ, the trigger egg activation in mammals, is present in a non-mammalian species. 於: Reproduction. 2005 ; 卷 130, 編號 2. 頁 157-163.
@article{447a65bb6f2f4d47946e2913edca5e7c,
title = "Phospholipase Cζ, the trigger egg activation in mammals, is present in a non-mammalian species",
abstract = "The activation of the egg to begin development into an embryo is triggered by a sperm-induced increase in intracellular egg Ca2+. There has been much controversy about how the sperm induces this fundamental developmental event, but recent studies suggest that, in mammals, egg activation is triggered by a testis-specific phospholipase C: PLCζ. Since the discovery of PLCζ, it has been unclear whether its role in triggering egg activation is common to all vertebrates, or is confined to mammals. Here, we demonstrate for the first time that PLCζ is present in a non-mammalian vertebrate. Using genomic and cDNA databases, we have identified the cDNA encoding a PLCζ orthologue in the domestic chicken that, like the mammalian isoforms, is a testis-specific gene. The chicken PLCζ cDNA is 2152bp in size and encodes an open reading frame of 639 amino acids. When injected into mouse oocytes, chicken PLCζ cRNA triggers Ca2+ oscillations, indicating that it has functional properties similar to those of mammalian PLCζ. Our findings suggest that PLCζ may have a universal role in triggering egg activation in vertebrates.",
author = "Kevin Coward and Ponting, {C. P.} and Chang, {H. Y.} and O. Hibbitt and P. Savolainen and Jones, {K. T.} and J. Parrington",
year = "2005",
month = "8",
doi = "10.1530/rep.1.00707",
language = "English",
volume = "130",
pages = "157--163",
journal = "Reproduction",
issn = "1470-1626",
publisher = "BioScientifica Ltd.",
number = "2",

}

TY - JOUR

T1 - Phospholipase Cζ, the trigger egg activation in mammals, is present in a non-mammalian species

AU - Coward, Kevin

AU - Ponting, C. P.

AU - Chang, H. Y.

AU - Hibbitt, O.

AU - Savolainen, P.

AU - Jones, K. T.

AU - Parrington, J.

PY - 2005/8

Y1 - 2005/8

N2 - The activation of the egg to begin development into an embryo is triggered by a sperm-induced increase in intracellular egg Ca2+. There has been much controversy about how the sperm induces this fundamental developmental event, but recent studies suggest that, in mammals, egg activation is triggered by a testis-specific phospholipase C: PLCζ. Since the discovery of PLCζ, it has been unclear whether its role in triggering egg activation is common to all vertebrates, or is confined to mammals. Here, we demonstrate for the first time that PLCζ is present in a non-mammalian vertebrate. Using genomic and cDNA databases, we have identified the cDNA encoding a PLCζ orthologue in the domestic chicken that, like the mammalian isoforms, is a testis-specific gene. The chicken PLCζ cDNA is 2152bp in size and encodes an open reading frame of 639 amino acids. When injected into mouse oocytes, chicken PLCζ cRNA triggers Ca2+ oscillations, indicating that it has functional properties similar to those of mammalian PLCζ. Our findings suggest that PLCζ may have a universal role in triggering egg activation in vertebrates.

AB - The activation of the egg to begin development into an embryo is triggered by a sperm-induced increase in intracellular egg Ca2+. There has been much controversy about how the sperm induces this fundamental developmental event, but recent studies suggest that, in mammals, egg activation is triggered by a testis-specific phospholipase C: PLCζ. Since the discovery of PLCζ, it has been unclear whether its role in triggering egg activation is common to all vertebrates, or is confined to mammals. Here, we demonstrate for the first time that PLCζ is present in a non-mammalian vertebrate. Using genomic and cDNA databases, we have identified the cDNA encoding a PLCζ orthologue in the domestic chicken that, like the mammalian isoforms, is a testis-specific gene. The chicken PLCζ cDNA is 2152bp in size and encodes an open reading frame of 639 amino acids. When injected into mouse oocytes, chicken PLCζ cRNA triggers Ca2+ oscillations, indicating that it has functional properties similar to those of mammalian PLCζ. Our findings suggest that PLCζ may have a universal role in triggering egg activation in vertebrates.

UR - http://www.scopus.com/inward/record.url?scp=23844457941&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=23844457941&partnerID=8YFLogxK

U2 - 10.1530/rep.1.00707

DO - 10.1530/rep.1.00707

M3 - Article

C2 - 16049153

AN - SCOPUS:23844457941

VL - 130

SP - 157

EP - 163

JO - Reproduction

JF - Reproduction

SN - 1470-1626

IS - 2

ER -