Pharmacological evaluation of several major ingredients of Chinese herbal medicines in human hepatoma Hep3B cells

C. C. Chou, S. L. Pan, C. M. Teng, J. H. Guh

研究成果: 雜誌貢獻文章同行評審

77 引文 斯高帕斯(Scopus)


Long-dan-tan (Chinese name) is one of the most common herbal medicines used by Chinese people with chronic liver disease. Accumulated anecdotal evidence suggests that Long-dan-tan may show a beneficial effect in patients with hepatocellular carcinoma. Long-dan-tan is made from five plants: Gentiana root, Scutellaria root, Gardenia fruit, Alisma rhizome, and Bupleurum root. In this study, we have examined the cytotoxic effects of the five major ingredients isolated from the above plants, i.e. gentiopicroside, baicalein, geniposide, alisol B acetate and saikosaponin-d, respectively, on human hepatoma Hep3B cells. Annexin V immunofluorescence detection, DNA fragmentation assays and FACScan analysis of propidium iodide-staining cells showed that gentiopicroside, baicalein, and geniposide had little effect, whereas alisol B acetate and saikosaponin-d profoundly induced apoptosis in Hep3B cells. Alisol B acetate, but not saikosaponin-d, induced G2/M arrest of the cell cycle as well as a significant increase in caspase-3 activity. Interestingly, baicalein by itself induced an increase in H2O2 generation and the subsequent NF-κB activation; furthermore, it effectively inhibited the transforming growth factor-β1 (TGF-β1)-induced caspase-3 activation and cell apoptosis. We suggest that alisol B acetate and saikosaponin-d induced cell apoptosis through the caspase-3-dependent and -independent pathways, respectively. Instead of inducing apoptosis, baicalein inhibits TGF-β1-induced apoptosis via increase in cellular H2O2 formation and NF-κB activation in human hepatoma Hep3B cells.
頁(從 - 到)403-412
期刊European Journal of Pharmaceutical Sciences
出版狀態已發佈 - 八月 2003

ASJC Scopus subject areas

  • Pharmaceutical Science

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