Pharmacokinetics and oral bioavailability of epimedin C after oral administration of epimedin C and Herba Epimedii extract in rats

Chia Jung Lee, Yu Tse Wu, Thomas Y. Hsueh, Lie Chwen Lin, Tung Hu Tsai

研究成果: 雜誌貢獻文章

14 引文 (Scopus)

摘要

Epimedin C, an ingredient of Herba Epimedii, has potential for treatment of cardiovascular disease and bone loss. However, there is still no sensitive analytical method to monitor epimedin C in biological samples. The goal of this study was to develop a sensitive and reliable method based on a LC-MS/MS for evaluating the pharmacokinetics of epimedin C after administration of Herba Epimedii in rat. Electrospray ionization in positive-ion mode and multiple reaction monitoring were used to identify and quantitate active components. Analytes were separated by a reverse-phase C18 column. Liquid-liquid extraction using ethyl acetate, evaporation and reconstitution was used to plasma sample preparation. Mass transition of precursor ion→product ion pairs were monitored at m/z 823.4→313.1 for epimedin C and m/z 237.1→178.9 for carbamazepine (internal standard). A calibration curve gave good linearity (r>0.999) over the concentration range 2.5-500ng/mL. Pharmacokinetic data demonstrated that there was rapid distribution and slow elimination after epimedin C administration (1mg/kg, i.v.). Oral bioavailabilities of epimedin C in the pure compound and in the Herba Epimedii were around 0.58% and 0.13%, respectively. The result suggests that other herbal ingredients of Herba Epimedii may suppress the oral bioavailability of epimedin C.

原文英語
頁(從 - 到)630-636
頁數7
期刊Biomedical Chromatography
28
發行號5
DOIs
出版狀態已發佈 - 2014
對外發佈Yes

指紋

Pharmacokinetics
Biological Availability
Oral Administration
Rats
Ions
Electrospray ionization
Liquid-Liquid Extraction
Distillation columns
Carbamazepine
Liquids
epimedin C
epimedii flavone
Calibration
Bone
Evaporation
Cardiovascular Diseases
Positive ions
Plasmas
Bone and Bones
Monitoring

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Molecular Biology
  • Drug Discovery
  • Pharmacology
  • Medicine(all)

引用此文

Pharmacokinetics and oral bioavailability of epimedin C after oral administration of epimedin C and Herba Epimedii extract in rats. / Lee, Chia Jung; Wu, Yu Tse; Hsueh, Thomas Y.; Lin, Lie Chwen; Tsai, Tung Hu.

於: Biomedical Chromatography, 卷 28, 編號 5, 2014, p. 630-636.

研究成果: 雜誌貢獻文章

Lee, Chia Jung ; Wu, Yu Tse ; Hsueh, Thomas Y. ; Lin, Lie Chwen ; Tsai, Tung Hu. / Pharmacokinetics and oral bioavailability of epimedin C after oral administration of epimedin C and Herba Epimedii extract in rats. 於: Biomedical Chromatography. 2014 ; 卷 28, 編號 5. 頁 630-636.
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abstract = "Epimedin C, an ingredient of Herba Epimedii, has potential for treatment of cardiovascular disease and bone loss. However, there is still no sensitive analytical method to monitor epimedin C in biological samples. The goal of this study was to develop a sensitive and reliable method based on a LC-MS/MS for evaluating the pharmacokinetics of epimedin C after administration of Herba Epimedii in rat. Electrospray ionization in positive-ion mode and multiple reaction monitoring were used to identify and quantitate active components. Analytes were separated by a reverse-phase C18 column. Liquid-liquid extraction using ethyl acetate, evaporation and reconstitution was used to plasma sample preparation. Mass transition of precursor ion→product ion pairs were monitored at m/z 823.4→313.1 for epimedin C and m/z 237.1→178.9 for carbamazepine (internal standard). A calibration curve gave good linearity (r>0.999) over the concentration range 2.5-500ng/mL. Pharmacokinetic data demonstrated that there was rapid distribution and slow elimination after epimedin C administration (1mg/kg, i.v.). Oral bioavailabilities of epimedin C in the pure compound and in the Herba Epimedii were around 0.58{\%} and 0.13{\%}, respectively. The result suggests that other herbal ingredients of Herba Epimedii may suppress the oral bioavailability of epimedin C.",
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AB - Epimedin C, an ingredient of Herba Epimedii, has potential for treatment of cardiovascular disease and bone loss. However, there is still no sensitive analytical method to monitor epimedin C in biological samples. The goal of this study was to develop a sensitive and reliable method based on a LC-MS/MS for evaluating the pharmacokinetics of epimedin C after administration of Herba Epimedii in rat. Electrospray ionization in positive-ion mode and multiple reaction monitoring were used to identify and quantitate active components. Analytes were separated by a reverse-phase C18 column. Liquid-liquid extraction using ethyl acetate, evaporation and reconstitution was used to plasma sample preparation. Mass transition of precursor ion→product ion pairs were monitored at m/z 823.4→313.1 for epimedin C and m/z 237.1→178.9 for carbamazepine (internal standard). A calibration curve gave good linearity (r>0.999) over the concentration range 2.5-500ng/mL. Pharmacokinetic data demonstrated that there was rapid distribution and slow elimination after epimedin C administration (1mg/kg, i.v.). Oral bioavailabilities of epimedin C in the pure compound and in the Herba Epimedii were around 0.58% and 0.13%, respectively. The result suggests that other herbal ingredients of Herba Epimedii may suppress the oral bioavailability of epimedin C.

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