The aim of the study was to develop a simple, rapid, and reliable technique to detect the pharmacokinetics and the residual of florfenicol (FFC) in Bester sturgeon, a cultured hybrid of Huso huso × Acipenser ruthenus by high performance liquid chromatography (HPLC) with UV detector. The results showed that the concentration-time course of the FFC was described by one-compartment model with 1st order absorption in the blood. The retention time of FFC was 7.3 min. The limit of detection of FFC concentration using our developed method was 0.01 mg kg−1 BW. After given single dose 10 mg kg−1 BW per day of FFC via oral administration for sturgeon, the main pharmacokinetic parameters in blood were found as following: Cmax (maximum concentration) was 10.9568 μg mL−1, Tmax (time to Cmax) was 10 h, AUC (area under the curve) was 251.064 μg × hr mL−1, t1/2 (half-life) was 12.1301 h, CL (total body clearance) was 0.0387 L kg−1, V/F (apparent volume of distribution) was 0.6777 L kg−1, and MRT (the mean residence time) was 23.7431 h. Additionally, the concentration-time course of the FFC was also described by two-compartment model with 1st order absorption in the muscle and liver. The recoveries of muscle and liver from sturgeon were 92–108% and 90–104%, respectively. Taken together, the results showed that the developed method technique may be a valuable tool was efficient in detecting the pharmacokinetics of FFC in Bester sturgeon.
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