Persistently elevated frequency of spontaneous mutations in progeny of CHO clones surviving X-irradiation: association with delayed reproductive death phenotype

Wushou P. Chang, John B. Little

研究成果: 雜誌貢獻文章同行評審

133 引文 斯高帕斯(Scopus)

摘要

Certain clonal progeny of Chinese hamster ovary (CHO) cells surviving X-irradiation demonstrate pleomorphic changes including a persistently decreased cloning efficiency, a dominant phenotype we have termed delayed reproductive death (Chang and Little, 1991, 1992b). We now report that cells from these progeny clones show a persistently elevated frequency of spontaneous mutations at the hprt locus for up to 95-100 population doubling post-irradiation. Mutant fractions as high as 10-3 were scored, more than two orders of magnitude higher than those observed in clonal progeny of non-irradiated cells studied in parallel. These results are discussed in terms of the hypothesis that radiation induces a type of genetic instability among some surviving cells that results in a heritable mutator phenotype, and that this instability may also be involved in the phenomenon of delayed reproductive death.

原文英語
頁(從 - 到)191-199
頁數9
期刊Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
270
發行號2
DOIs
出版狀態已發佈 - 十一月 16 1992
對外發佈

ASJC Scopus subject areas

  • 分子生物學
  • 健康、毒理學和誘變

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