TY - JOUR
T1 - Persistently elevated frequency of spontaneous mutations in progeny of CHO clones surviving X-irradiation
T2 - association with delayed reproductive death phenotype
AU - Chang, Wushou P.
AU - Little, John B.
PY - 1992/11/16
Y1 - 1992/11/16
N2 - Certain clonal progeny of Chinese hamster ovary (CHO) cells surviving X-irradiation demonstrate pleomorphic changes including a persistently decreased cloning efficiency, a dominant phenotype we have termed delayed reproductive death (Chang and Little, 1991, 1992b). We now report that cells from these progeny clones show a persistently elevated frequency of spontaneous mutations at the hprt locus for up to 95-100 population doubling post-irradiation. Mutant fractions as high as 10-3 were scored, more than two orders of magnitude higher than those observed in clonal progeny of non-irradiated cells studied in parallel. These results are discussed in terms of the hypothesis that radiation induces a type of genetic instability among some surviving cells that results in a heritable mutator phenotype, and that this instability may also be involved in the phenomenon of delayed reproductive death.
AB - Certain clonal progeny of Chinese hamster ovary (CHO) cells surviving X-irradiation demonstrate pleomorphic changes including a persistently decreased cloning efficiency, a dominant phenotype we have termed delayed reproductive death (Chang and Little, 1991, 1992b). We now report that cells from these progeny clones show a persistently elevated frequency of spontaneous mutations at the hprt locus for up to 95-100 population doubling post-irradiation. Mutant fractions as high as 10-3 were scored, more than two orders of magnitude higher than those observed in clonal progeny of non-irradiated cells studied in parallel. These results are discussed in terms of the hypothesis that radiation induces a type of genetic instability among some surviving cells that results in a heritable mutator phenotype, and that this instability may also be involved in the phenomenon of delayed reproductive death.
KW - Chinese hamster ovary (CHO) cells
KW - Delayed reproductive death
KW - Hprt
KW - Mutator
KW - X-Irradiation
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U2 - 10.1016/0027-5107(92)90130-T
DO - 10.1016/0027-5107(92)90130-T
M3 - Article
C2 - 1383736
AN - SCOPUS:0026523863
VL - 270
SP - 191
EP - 199
JO - Mutation Research
JF - Mutation Research
SN - 0027-5107
IS - 2
ER -