Penaeus monodon caspase is targeted by a white spot syndrome virus anti-apoptosis protein

Jiann Horng Leu, Hao Ching Wang, Guang Hsiung Kou, Chu Fang Lo

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44 引文 斯高帕斯(Scopus)

摘要

Caspases play a central and evolutionarily conserved role in mediating and executing apoptosis. Here, we report the cloning and characterization of a caspase from Penaeus monodon, Pm caspase. The full-length Pm caspase cDNA is 1386 bp, encoding a polypeptide of 304 amino acids with a calculated molecular mass of 34.3 kDa. BLASTP analysis against the NCBI nr database showed that Pm caspase is similar to insect effector caspases. RT-PCR analysis showed that Pm caspase mRNA is expressed in all examined tissues. When Pm caspase was overexpressed in SF-9 cells, the cells showed apoptotic morphological features, including the formation of apoptotic bodies and DNA ladders. The caspase-3 activity of Pm caspase was determined using the recombinant protein purified from Escherichia coli. Both RT-PCR and qRT-PCR analyses showed that the RNA levels of Pm caspase and P. monodon inhibitor of apoptosis protein (PmIAP) remained unchanged after white spot syndrome virus (WSSV) infection. We also used Pm caspase to show that WSSV449, an anti-apoptosis protein encoded by WSSV, is a direct caspase inhibitor.
原文英語
頁(從 - 到)476-486
頁數11
期刊Developmental and Comparative Immunology
32
發行號5
DOIs
出版狀態已發佈 - 2008
對外發佈Yes

ASJC Scopus subject areas

  • Developmental Biology
  • Immunology

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