PCR assay for the diagnosis of pneumococcal meningitis

Cheng Yu Wei, Terry B.J. Kuo, Hsin Yi Yau, Sy Yeu Yeh, Yeong Chu Tsai, Deeng Yih Lin, Ji Ying Lee, Feng Shu Chang, Wen Long Tsao

研究成果: 雜誌貢獻文章

摘要

Objective: Streptococcus pneumoniae is not an uncommon causative pathogen of acute bacterial meningitis, for which a slight delay in the management may result in death or serious sequelae. The traditional method of bacterial identification by culture requires at least 1-2 days, whereas use of PCR assay may allow much earlier recognition and confirmation of the bacteria. Materials and Methods: We designed a primer set targeting the amylomaltase gene (malM) of S. pneumoniae. Acute and convalescent cerebrospinal fluid (CSF) samples were collected from two patients of suspected acute bacterial meningitis. The acute CSF specimens were collected within the first hour the admission. The second specimens were collected during the convalescent stage after a 10-days treatment course with ceftriaxone. Polymerase chain reaction (PCR) assays were performed on the same day of sample collection. Results: The specificity and sensitivity of the PCR assay were demonstrated, and the latter shows that the latter could detect as few as 102 copies of molecules. In both patients the PCR assay identified the bacteria in the pre-treatment CSF samples, but not in the post-treatment specimens. All PCR assay results obtained in the first day were consistent with the culture results that were obtained on the fourth day of incubation. Conclusions: The use of PCR assays with the primer set targeting the malM gene would allow early recognition of the pneumococcal meningitis.
原文英語
頁(從 - 到)38-42
頁數5
期刊Acta Neurologica Taiwanica
12
發行號1
出版狀態已發佈 - 三月 2003
對外發佈Yes

指紋

Pneumococcal Meningitis
Polymerase Chain Reaction
Cerebrospinal Fluid
Bacterial Meningitides
Gene Targeting
Streptococcus pneumoniae
4 alpha-glucanotransferase
Bacteria
Ceftriaxone
Therapeutics
Sensitivity and Specificity

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology

引用此文

Wei, C. Y., Kuo, T. B. J., Yau, H. Y., Yeh, S. Y., Tsai, Y. C., Lin, D. Y., ... Tsao, W. L. (2003). PCR assay for the diagnosis of pneumococcal meningitis. Acta Neurologica Taiwanica, 12(1), 38-42.

PCR assay for the diagnosis of pneumococcal meningitis. / Wei, Cheng Yu; Kuo, Terry B.J.; Yau, Hsin Yi; Yeh, Sy Yeu; Tsai, Yeong Chu; Lin, Deeng Yih; Lee, Ji Ying; Chang, Feng Shu; Tsao, Wen Long.

於: Acta Neurologica Taiwanica, 卷 12, 編號 1, 03.2003, p. 38-42.

研究成果: 雜誌貢獻文章

Wei, CY, Kuo, TBJ, Yau, HY, Yeh, SY, Tsai, YC, Lin, DY, Lee, JY, Chang, FS & Tsao, WL 2003, 'PCR assay for the diagnosis of pneumococcal meningitis', Acta Neurologica Taiwanica, 卷 12, 編號 1, 頁 38-42.
Wei CY, Kuo TBJ, Yau HY, Yeh SY, Tsai YC, Lin DY 等. PCR assay for the diagnosis of pneumococcal meningitis. Acta Neurologica Taiwanica. 2003 3月;12(1):38-42.
Wei, Cheng Yu ; Kuo, Terry B.J. ; Yau, Hsin Yi ; Yeh, Sy Yeu ; Tsai, Yeong Chu ; Lin, Deeng Yih ; Lee, Ji Ying ; Chang, Feng Shu ; Tsao, Wen Long. / PCR assay for the diagnosis of pneumococcal meningitis. 於: Acta Neurologica Taiwanica. 2003 ; 卷 12, 編號 1. 頁 38-42.
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abstract = "Objective: Streptococcus pneumoniae is not an uncommon causative pathogen of acute bacterial meningitis, for which a slight delay in the management may result in death or serious sequelae. The traditional method of bacterial identification by culture requires at least 1-2 days, whereas use of PCR assay may allow much earlier recognition and confirmation of the bacteria. Materials and Methods: We designed a primer set targeting the amylomaltase gene (malM) of S. pneumoniae. Acute and convalescent cerebrospinal fluid (CSF) samples were collected from two patients of suspected acute bacterial meningitis. The acute CSF specimens were collected within the first hour the admission. The second specimens were collected during the convalescent stage after a 10-days treatment course with ceftriaxone. Polymerase chain reaction (PCR) assays were performed on the same day of sample collection. Results: The specificity and sensitivity of the PCR assay were demonstrated, and the latter shows that the latter could detect as few as 102 copies of molecules. In both patients the PCR assay identified the bacteria in the pre-treatment CSF samples, but not in the post-treatment specimens. All PCR assay results obtained in the first day were consistent with the culture results that were obtained on the fourth day of incubation. Conclusions: The use of PCR assays with the primer set targeting the malM gene would allow early recognition of the pneumococcal meningitis.",
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AU - Wei, Cheng Yu

AU - Kuo, Terry B.J.

AU - Yau, Hsin Yi

AU - Yeh, Sy Yeu

AU - Tsai, Yeong Chu

AU - Lin, Deeng Yih

AU - Lee, Ji Ying

AU - Chang, Feng Shu

AU - Tsao, Wen Long

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N2 - Objective: Streptococcus pneumoniae is not an uncommon causative pathogen of acute bacterial meningitis, for which a slight delay in the management may result in death or serious sequelae. The traditional method of bacterial identification by culture requires at least 1-2 days, whereas use of PCR assay may allow much earlier recognition and confirmation of the bacteria. Materials and Methods: We designed a primer set targeting the amylomaltase gene (malM) of S. pneumoniae. Acute and convalescent cerebrospinal fluid (CSF) samples were collected from two patients of suspected acute bacterial meningitis. The acute CSF specimens were collected within the first hour the admission. The second specimens were collected during the convalescent stage after a 10-days treatment course with ceftriaxone. Polymerase chain reaction (PCR) assays were performed on the same day of sample collection. Results: The specificity and sensitivity of the PCR assay were demonstrated, and the latter shows that the latter could detect as few as 102 copies of molecules. In both patients the PCR assay identified the bacteria in the pre-treatment CSF samples, but not in the post-treatment specimens. All PCR assay results obtained in the first day were consistent with the culture results that were obtained on the fourth day of incubation. Conclusions: The use of PCR assays with the primer set targeting the malM gene would allow early recognition of the pneumococcal meningitis.

AB - Objective: Streptococcus pneumoniae is not an uncommon causative pathogen of acute bacterial meningitis, for which a slight delay in the management may result in death or serious sequelae. The traditional method of bacterial identification by culture requires at least 1-2 days, whereas use of PCR assay may allow much earlier recognition and confirmation of the bacteria. Materials and Methods: We designed a primer set targeting the amylomaltase gene (malM) of S. pneumoniae. Acute and convalescent cerebrospinal fluid (CSF) samples were collected from two patients of suspected acute bacterial meningitis. The acute CSF specimens were collected within the first hour the admission. The second specimens were collected during the convalescent stage after a 10-days treatment course with ceftriaxone. Polymerase chain reaction (PCR) assays were performed on the same day of sample collection. Results: The specificity and sensitivity of the PCR assay were demonstrated, and the latter shows that the latter could detect as few as 102 copies of molecules. In both patients the PCR assay identified the bacteria in the pre-treatment CSF samples, but not in the post-treatment specimens. All PCR assay results obtained in the first day were consistent with the culture results that were obtained on the fourth day of incubation. Conclusions: The use of PCR assays with the primer set targeting the malM gene would allow early recognition of the pneumococcal meningitis.

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