Partial decellularized scaffold combined with autologous nasal epithelial cell sheet for tracheal tissue engineering

Luong Huu Dang, Shih Han Hung, Yuan Tseng, Ly Xuan Quang, Nhi Thao Ngoc Le, Chia Lang Fang, How Tseng

研究成果: 雜誌貢獻文章同行評審

摘要

Decellularization has emerged as a potential solution for tracheal replacement. As a fully decellularized graft failed to achieve its purposes, the de-epithelialization partial decellularization protocol appeared to be a promising approach for fabricating scaffolds with preserved mechanical properties and few immune rejection responses after transplantation. Nevertheless, a lack of appropriate concurrent epithelialization treatment can lead to luminal stenosis of the transplant and impede its eventual success. To improve re-epithelialization, autologous nasal epithelial cell sheets generated by our cell sheet engineering platform were utilized in this study under an in vivo rabbit model. The newly created cell sheets have an intact and transplantable appearance, with their specific characteristics of airway epithelial origin being highly expressed upon histological and immunohistochemical analysis. Subsequently, those cell sheets were incorporated with a partially decellularized tracheal graft for autograft transplantation under tracheal partial resection models. The preliminary results two months post operation demonstrated that the transplanted patches appeared to be wholly integrated into the host trachea with adequate healing of the luminal surface, which was confirmed via endoscopic and histologic evaluations. The satisfactory result of this hybrid scaffold protocol could serve as a potential solution for tracheal reconstructions in the future.
原文英語
文章編號10322
期刊International journal of molecular sciences
22
發行號19
DOIs
出版狀態已發佈 - 10月 1 2021

ASJC Scopus subject areas

  • 催化
  • 分子生物學
  • 光譜
  • 電腦科學應用
  • 物理與理論化學
  • 有機化學
  • 無機化學

指紋

深入研究「Partial decellularized scaffold combined with autologous nasal epithelial cell sheet for tracheal tissue engineering」主題。共同形成了獨特的指紋。

引用此