Paradoxical effect of GM-CSF gene transfer on the tumorigenicity and immunogenicity of murine tumors

Jie Wang, Wen K. Yang, Yili Yang, Sung Jan Wei, Den Mei Yang, Jacqueline Whang-Peng, Yuh Min Chen, Kai Li Ting, Chou Chik Ting

研究成果: 雜誌貢獻文章

10 引文 斯高帕斯(Scopus)

摘要

The effect of granulocyte/macrophage colony-stimulating factor (GM-CSF) gene transfer on the tumorigenicity and immunogenicity of 2 different murine tumor lines was determined. Transduction of B16 melanoma cells with the GM- CSF gene rendered the cells more immunogenic. In contrast, transduction of NG4TL4 fibrosarcoma in FVB/N mice (NG) with the GM-CSF gene showed increased tumorigenicity in a high producer line (NG-MGh). The parent NG or NG-MG cells induced the same level of cytotoxic T-lymphocyte (CTL) response and the same magnitude of tumor transplantation immunity. However, the proliferation of the NG-MGh cells was increased 2- to 10-fold. There was no increase in apoptosis in the NG cells and there was no increase of NG-MGh cells in S- phase, hence the increase of the proliferative activity appeared to be indeed inherent to the cells. Mixing the splenocytes from the NG-MGh tumor bearers with the NG tumor cells did not increase tumorigenicity but totally inhibited the growth of the NG tumor, indicating that suppressor cells were not present. Mixing 10,000 rad X-irradiated NG-MGh cells with viable NG tumor cells resulted in 3- to 10-fold increased NG tumor growth rate. The in vitro proliferation of NG cells was increased by adding both GM-CSFs and macrophages and not by either one alone, suggesting that interaction between macrophages and GM-CSFs resulted in the production of tumor growth enhancing factor(s). Our findrags suggest that trensduction of NG tumor cells with the GM-CSF gene increases tumorigenicity, which is attributed both to an increased inherent proliferative ability of the tumor cells and to the in vivo production of a tumor growth enhancing factor(s) at the tumor site.
原文英語
頁(從 - 到)459-466
頁數8
期刊International Journal of Cancer
75
發行號3
DOIs
出版狀態已發佈 - 一月 30 1998
對外發佈Yes

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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