Oxidative stress-induced cellular senescence desensitizes cell growth and migration of vascular smooth muscle cells through down-regulation of platelet-derived growth factor receptor-beta

Chun Hsu Pan, Chang Jui Chen, Chun Ming Shih, Ming Fu Wang, Jie Yu Wang, Chieh Hsi Wu

研究成果: 雜誌貢獻文章

摘要

The relationship between aging and restenosis are unclear. The purposes of this study were to investigate the possible pathological role and mechanism of aging on formation of restenosis. Our data indicated that cell proliferation and migration of the oxidative stress-induced senescent vascular smooth muscle cells were obviously desensitized to stimulation by platelet-derived growth factor (PDGF)-BB, which may have been caused by suppression of promoter activity, transcription, translation, and activation levels of PDGF receptor (PDGFR)-β. The analyzed data obtained from the binding array of transcription factors (TFs) showed that binding levels of eighteen TFs on the PDGFR-β promoter region (-523 to -1) were significantly lower in senescent cells compared to those of non-senescent cells. Among these TFs, the bioinformatics prediction suggested that the putative binding sites of ten TFs were found in this promoter region. Of these, transcriptional levels of seven TFs were markedly reduced in senescent cells. The clinical data showed that the proportion of restenosis was relatively lower in the older group than that in the younger group. Our study results suggested that a PDGFR-β-mediated pathway was suppressed in aging cells, and our clinical data showed that age and the vascular status were slightly negatively correlated in overall participants.
原文英語
頁(從 - 到)8085-8102
頁數18
期刊Aging
11
發行號19
DOIs
出版狀態已發佈 - 一月 1 2019

指紋

Platelet-Derived Growth Factor beta Receptor
Cell Aging
Vascular Smooth Muscle
Smooth Muscle Myocytes
Cell Movement
Oxidative Stress
Transcription Factors
Down-Regulation
Platelet-Derived Growth Factor Receptors
Growth
Genetic Promoter Regions
Computational Biology
Transcriptional Activation
Blood Vessels
Binding Sites
Cell Proliferation

ASJC Scopus subject areas

  • Ageing
  • Cell Biology

引用此文

@article{b9caa4da766e48adac748d0137eadcc9,
title = "Oxidative stress-induced cellular senescence desensitizes cell growth and migration of vascular smooth muscle cells through down-regulation of platelet-derived growth factor receptor-beta",
abstract = "The relationship between aging and restenosis are unclear. The purposes of this study were to investigate the possible pathological role and mechanism of aging on formation of restenosis. Our data indicated that cell proliferation and migration of the oxidative stress-induced senescent vascular smooth muscle cells were obviously desensitized to stimulation by platelet-derived growth factor (PDGF)-BB, which may have been caused by suppression of promoter activity, transcription, translation, and activation levels of PDGF receptor (PDGFR)-β. The analyzed data obtained from the binding array of transcription factors (TFs) showed that binding levels of eighteen TFs on the PDGFR-β promoter region (-523 to -1) were significantly lower in senescent cells compared to those of non-senescent cells. Among these TFs, the bioinformatics prediction suggested that the putative binding sites of ten TFs were found in this promoter region. Of these, transcriptional levels of seven TFs were markedly reduced in senescent cells. The clinical data showed that the proportion of restenosis was relatively lower in the older group than that in the younger group. Our study results suggested that a PDGFR-β-mediated pathway was suppressed in aging cells, and our clinical data showed that age and the vascular status were slightly negatively correlated in overall participants.",
keywords = "Aging, Cellular senescence, Platelet-derived growth factor, Restenosis, Vascular smooth muscle cells",
author = "Pan, {Chun Hsu} and Chen, {Chang Jui} and Shih, {Chun Ming} and Wang, {Ming Fu} and Wang, {Jie Yu} and Wu, {Chieh Hsi}",
year = "2019",
month = "1",
day = "1",
doi = "10.18632/aging.102270",
language = "English",
volume = "11",
pages = "8085--8102",
journal = "Aging",
issn = "0002-0966",
publisher = "US Administration on Aging",
number = "19",

}

TY - JOUR

T1 - Oxidative stress-induced cellular senescence desensitizes cell growth and migration of vascular smooth muscle cells through down-regulation of platelet-derived growth factor receptor-beta

AU - Pan, Chun Hsu

AU - Chen, Chang Jui

AU - Shih, Chun Ming

AU - Wang, Ming Fu

AU - Wang, Jie Yu

AU - Wu, Chieh Hsi

PY - 2019/1/1

Y1 - 2019/1/1

N2 - The relationship between aging and restenosis are unclear. The purposes of this study were to investigate the possible pathological role and mechanism of aging on formation of restenosis. Our data indicated that cell proliferation and migration of the oxidative stress-induced senescent vascular smooth muscle cells were obviously desensitized to stimulation by platelet-derived growth factor (PDGF)-BB, which may have been caused by suppression of promoter activity, transcription, translation, and activation levels of PDGF receptor (PDGFR)-β. The analyzed data obtained from the binding array of transcription factors (TFs) showed that binding levels of eighteen TFs on the PDGFR-β promoter region (-523 to -1) were significantly lower in senescent cells compared to those of non-senescent cells. Among these TFs, the bioinformatics prediction suggested that the putative binding sites of ten TFs were found in this promoter region. Of these, transcriptional levels of seven TFs were markedly reduced in senescent cells. The clinical data showed that the proportion of restenosis was relatively lower in the older group than that in the younger group. Our study results suggested that a PDGFR-β-mediated pathway was suppressed in aging cells, and our clinical data showed that age and the vascular status were slightly negatively correlated in overall participants.

AB - The relationship between aging and restenosis are unclear. The purposes of this study were to investigate the possible pathological role and mechanism of aging on formation of restenosis. Our data indicated that cell proliferation and migration of the oxidative stress-induced senescent vascular smooth muscle cells were obviously desensitized to stimulation by platelet-derived growth factor (PDGF)-BB, which may have been caused by suppression of promoter activity, transcription, translation, and activation levels of PDGF receptor (PDGFR)-β. The analyzed data obtained from the binding array of transcription factors (TFs) showed that binding levels of eighteen TFs on the PDGFR-β promoter region (-523 to -1) were significantly lower in senescent cells compared to those of non-senescent cells. Among these TFs, the bioinformatics prediction suggested that the putative binding sites of ten TFs were found in this promoter region. Of these, transcriptional levels of seven TFs were markedly reduced in senescent cells. The clinical data showed that the proportion of restenosis was relatively lower in the older group than that in the younger group. Our study results suggested that a PDGFR-β-mediated pathway was suppressed in aging cells, and our clinical data showed that age and the vascular status were slightly negatively correlated in overall participants.

KW - Aging

KW - Cellular senescence

KW - Platelet-derived growth factor

KW - Restenosis

KW - Vascular smooth muscle cells

UR - http://www.scopus.com/inward/record.url?scp=85073646325&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85073646325&partnerID=8YFLogxK

U2 - 10.18632/aging.102270

DO - 10.18632/aging.102270

M3 - Article

C2 - 31584878

AN - SCOPUS:85073646325

VL - 11

SP - 8085

EP - 8102

JO - Aging

JF - Aging

SN - 0002-0966

IS - 19

ER -