Oxidative DNA damage in human peripheral leukocytes induced by massive aerobic exercise

Kelvin Tsai, Tai Ger Hsu, Kuang Ming Hsu, Hu Cheng, Tsung Yun Liu, Chen Fu Hsu, Chi Woon Kong

研究成果: 雜誌貢獻文章

178 引文 斯高帕斯(Scopus)

摘要

Reactive oxygen species produced during vigorous exercise may permeate into cell nuclei and induce oxidative DNA damage, but the supporting evidence is still lacking. By using a 42 km marathon race as a model of massive aerobic exercise, we demonstrated a significant degree of unrepaired DNA base oxidation in peripheral immunocompetent cells, despite a concurrent increase in the urinary excretion of 8-hydroxy-2′-deoxyguanosine. Single cell gel electrophoresis with the incorporation of lesion-specific endonucleases further revealed that oxidized pyrimidines (endonuclease III-sensitive sites) contributed to most of the postexercise nucleotide oxidation. The oxidative DNA damage correlated significantly with plasma levels of creatinine kinase and lipid peroxidation metabolites, and lasted for more than 1 week following the race. This phenomenon may be one of the mechanisms behind the immune dysfunctions after exhaustive exercise.
原文英語
頁(從 - 到)1465-1472
頁數8
期刊Free Radical Biology and Medicine
31
發行號11
DOIs
出版狀態已發佈 - 十二月 1 2001
對外發佈Yes

ASJC Scopus subject areas

  • Medicine(all)
  • Toxicology
  • Clinical Biochemistry

指紋 深入研究「Oxidative DNA damage in human peripheral leukocytes induced by massive aerobic exercise」主題。共同形成了獨特的指紋。

  • 引用此