摘要
Previous biochemical studies have suggested a role for bacterial DNA topoisomerase (TOPO) I in the suppression of R-loop formation during transcription. In this report, we present several pieces of genetic evidence to support a model in which R-loop formation is dynamically regulated during transcription by activities of multiple DNA TOPOs and RNase H. In addition, our results suggest that events leading to the serious growth problems in the absence of DNA TOPO I are linked to R-loop formation. We show that the overexpression of RNase H, an enzyme that degrades the RNA moiety of an R loop, can partially compensate for the absence of DNA TOPO I. We also note that a defect in DNA gyrase can correct several phenotypes associated with a mutation in the rnhA gene, which encodes the major RNase H activity. In addition, we found that a combination of topA and rnhA mutations is lethal.
| 原文 | 英語 |
|---|---|
| 頁（從 - 到） | 3526-3530 |
| 頁數 | 5 |
| 期刊 | Proceedings of the National Academy of Sciences of the United States of America |
| 卷 | 92 |
| 發行號 | 8 |
| 出版狀態 | 已發佈 - 1995 |
| 對外發佈 | Yes |
指紋
ASJC Scopus subject areas
- General
- Genetics
引用此文
Overexpression of RNase H partially complements the growth defect of an Escherichia coli ΔtopA mutant : R-loop formation is a major problem in the absence of DNA topoisomerase I. / Drolet, M.; Phoenix, P.; Menzel, R.; Masse, E.; Liu, Leroy-Fong; Crouch, R. J.
於: Proceedings of the National Academy of Sciences of the United States of America, 卷 92, 編號 8, 1995, p. 3526-3530.研究成果: 雜誌貢獻 › 文章
}
TY - JOUR
T1 - Overexpression of RNase H partially complements the growth defect of an Escherichia coli ΔtopA mutant
T2 - R-loop formation is a major problem in the absence of DNA topoisomerase I
AU - Drolet, M.
AU - Phoenix, P.
AU - Menzel, R.
AU - Masse, E.
AU - Liu, Leroy-Fong
AU - Crouch, R. J.
PY - 1995
Y1 - 1995
N2 - Previous biochemical studies have suggested a role for bacterial DNA topoisomerase (TOPO) I in the suppression of R-loop formation during transcription. In this report, we present several pieces of genetic evidence to support a model in which R-loop formation is dynamically regulated during transcription by activities of multiple DNA TOPOs and RNase H. In addition, our results suggest that events leading to the serious growth problems in the absence of DNA TOPO I are linked to R-loop formation. We show that the overexpression of RNase H, an enzyme that degrades the RNA moiety of an R loop, can partially compensate for the absence of DNA TOPO I. We also note that a defect in DNA gyrase can correct several phenotypes associated with a mutation in the rnhA gene, which encodes the major RNase H activity. In addition, we found that a combination of topA and rnhA mutations is lethal.
AB - Previous biochemical studies have suggested a role for bacterial DNA topoisomerase (TOPO) I in the suppression of R-loop formation during transcription. In this report, we present several pieces of genetic evidence to support a model in which R-loop formation is dynamically regulated during transcription by activities of multiple DNA TOPOs and RNase H. In addition, our results suggest that events leading to the serious growth problems in the absence of DNA TOPO I are linked to R-loop formation. We show that the overexpression of RNase H, an enzyme that degrades the RNA moiety of an R loop, can partially compensate for the absence of DNA TOPO I. We also note that a defect in DNA gyrase can correct several phenotypes associated with a mutation in the rnhA gene, which encodes the major RNase H activity. In addition, we found that a combination of topA and rnhA mutations is lethal.
UR - http://www.scopus.com/inward/record.url?scp=0028966185&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028966185&partnerID=8YFLogxK
M3 - Article
C2 - 7536935
AN - SCOPUS:0028966185
VL - 92
SP - 3526
EP - 3530
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 8
ER -