Overexpression of Notch ligand Delta-like-1 by dendritic cells enhances their immunoregulatory capacity and exerts antiallergic effects on Th2-mediated allergic asthma in mice

Chen Chen Lee, Chu Lun Lin, Sy Jye Leu, Yueh Lun Lee

研究成果: 雜誌貢獻 › 文章

3 引文 (Scopus)

摘要

Dendritic cells (DCs) are professional antigen-presenting cells, and Notch ligand Delta-like-1 (DLL1) on DCs was implicated in type 1T helper (Th1) differentiation. In this study, we produced genetically engineered bone marrow-derived DCs that expressed DLL1 (DLL1-DCs) by adenoviral transduction. DLL1-DCs exerted a fully mature phenotype, and had positive effects on expression levels of interleukin (IL)-12 and costimulatory molecules. Coculture of allogeneic T cells with ovalbumin (OVA)-pulsed DLL1-DCs enhanced T cell proliferative responses and promoted Th1 cell differentiation. Furthermore, adoptive transfer of OVA-stimulated DLL1-DCs into asthmatic mice alleviated the cardinal features of allergic asthma, including immunoglobulin E (IgE) production, airway hyperresponsiveness (AHR), airway inflammation, and production of Th2-type cytokines. Notably, enhanced levels of the Th1-biased IgG2a response and interferon (IFN)-γ production were observed in these mice. Taken together, these data indicate that DLL1-DCs promoted Th1 cell development to alter the Th1/Th2 ratio and ameliorate Th2-mediated allergic asthma in mice.
原文英語
頁（從 - 到）58-67
期刊Clinical Immunology
卷187
DOIs
出版狀態已發佈 - 二月 2018

指紋

Anti-Allergic Agents
Dendritic Cells
Asthma
Ligands
Th1 Cells
Ovalbumin
T-Lymphocytes
Adoptive Transfer
Antigen-Presenting Cells
Interleukin-12
Coculture Techniques
Interferons
Immunoglobulin E
Cell Differentiation
Bone Marrow
Cytokines
Inflammation
Phenotype

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

引用此文

Overexpression of Notch ligand Delta-like-1 by dendritic cells enhances their immunoregulatory capacity and exerts antiallergic effects on Th2-mediated allergic asthma in mice. / Lee, Chen Chen; Lin, Chu Lun; Leu, Sy Jye; Lee, Yueh Lun.

於: Clinical Immunology, 卷 187, 02.2018, p. 58-67.

研究成果: 雜誌貢獻 › 文章

Lee, CC, Lin, CL, Leu, SJ & Lee, YL 2018, 'Overexpression of Notch ligand Delta-like-1 by dendritic cells enhances their immunoregulatory capacity and exerts antiallergic effects on Th2-mediated allergic asthma in mice', Clinical Immunology, 卷 187, 頁 58-67. https://doi.org/10.1016/j.clim.2017.10.005
Lee CC, Lin CL, Leu SJ, Lee YL. Overexpression of Notch ligand Delta-like-1 by dendritic cells enhances their immunoregulatory capacity and exerts antiallergic effects on Th2-mediated allergic asthma in mice. Clinical Immunology. 2018 2月;187:58-67. https://doi.org/10.1016/j.clim.2017.10.005
Lee, Chen Chen ; Lin, Chu Lun ; Leu, Sy Jye ; Lee, Yueh Lun. / Overexpression of Notch ligand Delta-like-1 by dendritic cells enhances their immunoregulatory capacity and exerts antiallergic effects on Th2-mediated allergic asthma in mice. 於: Clinical Immunology. 2018 ; 卷 187. 頁 58-67.
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