摘要
原文 | 英語 |
---|---|
主出版物標題 | Methods in Molecular Biology |
發行者 | Humana Press Inc. |
頁面 | 27-42 |
頁數 | 16 |
DOIs | |
出版狀態 | 已發佈 - 一月 1 2019 |
出版系列
名字 | Methods in Molecular Biology |
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卷 | 1895 |
ISSN(列印) | 1064-3745 |
指紋
ASJC Scopus subject areas
- Molecular Biology
- Genetics
引用此文
Osteonectin promoter-mediated suicide gene therapy of prostate cancer. / Hsiao, Wan Chi; Sung, Shian Ying; Chung, Leland W.K.; Hsieh, Chia Ling.
Methods in Molecular Biology. Humana Press Inc., 2019. p. 27-42 (Methods in Molecular Biology; 卷 1895).研究成果: 書貢獻/報告類型 › 章節
}
TY - CHAP
T1 - Osteonectin promoter-mediated suicide gene therapy of prostate cancer
AU - Hsiao, Wan Chi
AU - Sung, Shian Ying
AU - Chung, Leland W.K.
AU - Hsieh, Chia Ling
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Suicide gene therapy using the herpes simplex virus thymidine kinase (HSV-tk) gene, combined with the prodrug ganciclovir (GCV) medication, is a promising approach for the treatment of malignant tumors, including prostate cancer. The success of this therapeutic strategy requires tissue- or tumor-specific gene expression and efficient gene delivery. In this chapter, we describe the experimental protocols of key methodologies, including promoter construction, reporter assay, adenoviral vector construction and preparation, HSV-tk enzymatic assay and cytotoxicity assay to evaluate the specificity and efficacy of osteonectin promoter-mediated HSV-tk/GCV suicide gene therapy of prostate cancer.
AB - Suicide gene therapy using the herpes simplex virus thymidine kinase (HSV-tk) gene, combined with the prodrug ganciclovir (GCV) medication, is a promising approach for the treatment of malignant tumors, including prostate cancer. The success of this therapeutic strategy requires tissue- or tumor-specific gene expression and efficient gene delivery. In this chapter, we describe the experimental protocols of key methodologies, including promoter construction, reporter assay, adenoviral vector construction and preparation, HSV-tk enzymatic assay and cytotoxicity assay to evaluate the specificity and efficacy of osteonectin promoter-mediated HSV-tk/GCV suicide gene therapy of prostate cancer.
KW - Adenoviral vector
KW - HSV-tk enzymatic assay
KW - Osteonectin promoter construction
KW - Prostate cancer
KW - Suicide gene therapy
UR - http://www.scopus.com/inward/record.url?scp=85058589961&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85058589961&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-8922-5_3
DO - 10.1007/978-1-4939-8922-5_3
M3 - Chapter
C2 - 30539527
AN - SCOPUS:85058589961
T3 - Methods in Molecular Biology
SP - 27
EP - 42
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -