Osteonectin promoter-mediated suicide gene therapy of prostate cancer

Wan Chi Hsiao, Shian Ying Sung, Leland W.K. Chung, Chia Ling Hsieh

研究成果: 書貢獻/報告類型章節

1 引文 斯高帕斯(Scopus)

摘要

Suicide gene therapy using the herpes simplex virus thymidine kinase (HSV-tk) gene, combined with the prodrug ganciclovir (GCV) medication, is a promising approach for the treatment of malignant tumors, including prostate cancer. The success of this therapeutic strategy requires tissue- or tumor-specific gene expression and efficient gene delivery. In this chapter, we describe the experimental protocols of key methodologies, including promoter construction, reporter assay, adenoviral vector construction and preparation, HSV-tk enzymatic assay and cytotoxicity assay to evaluate the specificity and efficacy of osteonectin promoter-mediated HSV-tk/GCV suicide gene therapy of prostate cancer.
原文英語
主出版物標題Methods in Molecular Biology
發行者Humana Press Inc.
頁面27-42
頁數16
DOIs
出版狀態已發佈 - 一月 1 2019

出版系列

名字Methods in Molecular Biology
1895
ISSN(列印)1064-3745

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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