Osteonectin promoter-mediated suicide gene therapy of prostate cancer

Wan Chi Hsiao, Shian Ying Sung, Leland W.K. Chung, Chia Ling Hsieh

研究成果: 書貢獻/報告類型章節

摘要

Suicide gene therapy using the herpes simplex virus thymidine kinase (HSV-tk) gene, combined with the prodrug ganciclovir (GCV) medication, is a promising approach for the treatment of malignant tumors, including prostate cancer. The success of this therapeutic strategy requires tissue- or tumor-specific gene expression and efficient gene delivery. In this chapter, we describe the experimental protocols of key methodologies, including promoter construction, reporter assay, adenoviral vector construction and preparation, HSV-tk enzymatic assay and cytotoxicity assay to evaluate the specificity and efficacy of osteonectin promoter-mediated HSV-tk/GCV suicide gene therapy of prostate cancer.
原文英語
主出版物標題Methods in Molecular Biology
發行者Humana Press Inc.
頁面27-42
頁數16
DOIs
出版狀態已發佈 - 一月 1 2019

出版系列

名字Methods in Molecular Biology
1895
ISSN(列印)1064-3745

指紋

Osteonectin
Thymidine Kinase
Simplexvirus
Genetic Therapy
Suicide
Prostatic Neoplasms
Ganciclovir
Prodrugs
Enzyme Assays
Genes
Neoplasms
Gene Expression
Therapeutics

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

引用此文

Hsiao, W. C., Sung, S. Y., Chung, L. W. K., & Hsieh, C. L. (2019). Osteonectin promoter-mediated suicide gene therapy of prostate cancer. 於 Methods in Molecular Biology (頁 27-42). (Methods in Molecular Biology; 卷 1895). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-8922-5_3

Osteonectin promoter-mediated suicide gene therapy of prostate cancer. / Hsiao, Wan Chi; Sung, Shian Ying; Chung, Leland W.K.; Hsieh, Chia Ling.

Methods in Molecular Biology. Humana Press Inc., 2019. p. 27-42 (Methods in Molecular Biology; 卷 1895).

研究成果: 書貢獻/報告類型章節

Hsiao, WC, Sung, SY, Chung, LWK & Hsieh, CL 2019, Osteonectin promoter-mediated suicide gene therapy of prostate cancer. 於 Methods in Molecular Biology. Methods in Molecular Biology, 卷 1895, Humana Press Inc., 頁 27-42. https://doi.org/10.1007/978-1-4939-8922-5_3
Hsiao WC, Sung SY, Chung LWK, Hsieh CL. Osteonectin promoter-mediated suicide gene therapy of prostate cancer. 於 Methods in Molecular Biology. Humana Press Inc. 2019. p. 27-42. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-8922-5_3
Hsiao, Wan Chi ; Sung, Shian Ying ; Chung, Leland W.K. ; Hsieh, Chia Ling. / Osteonectin promoter-mediated suicide gene therapy of prostate cancer. Methods in Molecular Biology. Humana Press Inc., 2019. 頁 27-42 (Methods in Molecular Biology).
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