13 引文 斯高帕斯(Scopus)

摘要

Efficient nonviral oral gene delivery offers an attractive modality for chronic protein replacement therapy. Herein, the oral delivery of insulin gene is reported by a nonviral vector comprising a copolymer with a high degree of substitution of branched polyethylenimine on chitosan (CS-g-bPEI). Protecting the plasmid from gastric acidic degradation and facilitating transport across the gut epithelium, the CS-g-bPEI/insulin plasmid DNA nanoparticles (NPs) can achieve systemic transgene expression for days. A single dose of orally administered NPs (600 µg plasmid insulin (pINS)) to diabetic mice can protect the animals from hyperglycemia for more than 10 d. Three repeated administrations spaced over a 10 d interval produce similar glucose-lowering results with no hepatotoxicity detected. Positron-emission-tomography and computed-tomography images also confirm the glucose utilization by muscle cells. While this work suggests the feasibility of basal therapy for diabetes mellitus, its significance lies in the demonstration of a nonviral oral gene delivery system that can impact chronic protein replacement therapy and DNA vaccination.
原文英語
文章編號1701079
期刊Advanced Science
5
發行號8
DOIs
出版狀態已發佈 - 八月 1 2018

ASJC Scopus subject areas

  • 醫藥(雜項)
  • 化學工程 (全部)
  • 材料科學(全部)
  • 生物化學、遺傳與分子生物學(雜項)
  • 工程 (全部)
  • 物理與天文學 (全部)

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