Oral gene delivery with chitosan-DNA nanoparticles generates immunologic protection in a murine model of peanut allergy

Krishnendu Roy, Hai Quan Mao, Shau Ku Huang, Kam W. Leong

研究成果: 雜誌貢獻文章同行評審

1062 引文 斯高帕斯(Scopus)

摘要

Food allergy is a common and often fatal disease with no effective treatment. We describe here a new immunoprophylactic strategy using oral allergen-gene immunization to modulate peanut antigen-induced murine anaphylactic responses. Oral administration of DNA nanoparticles synthesized by complexing plasmid DNA with chitosan, a natural biocompatible polysaccharide, resulted in transduced gene expression in the intestinal epithelium. Mice receiving nanoparticles containing a dominant peanut allergen gene (pCMVArah2) produced secretory IgA and serum IgG2a. Compared with non-immunized mice or mice treated with 'naked' DNA, mice immunized with nanoparticles showed a substantial reduction in allergen-induced anaphylaxis associated with reduced levels of IgE, plasma histamine and vascular leakage. These results demonstrate that oral allergen-gene immunization with chitosan- DNA nanoparticles is effective in modulating murine anaphylactic responses, and indicate its prophylactic utility in treating food allergy.

原文英語
頁(從 - 到)387-391
頁數5
期刊Nature Medicine
5
發行號4
DOIs
出版狀態已發佈 - 4月 1999
對外發佈

ASJC Scopus subject areas

  • 生物化學、遺傳與分子生物學 (全部)

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