Optical mapping of myocardial reactive oxygen species production throughout the reperfusion of global ischemia

Long Sheng Lu, Yen Bin Liu, Chia Wei Sun, Lung Chun Lin, Ming Jai Su, Chau Chung Wu

研究成果: 雜誌貢獻文章同行評審

7 引文 斯高帕斯(Scopus)

摘要

Reactive oxygen species (ROS) are short-lived, highly reactive chemical entities that play significant roles in all levels of biology. However, their measurement requires destructive preparation, thereby limiting the continuous measurement of ROS in a living tissue. We develop an optical mapping system to visualize ROS production in an isolated and perfused rat heart. By staining the heart with dihydroethidium (DHE), a 532-nm laser beam is directed to the epicardial surface, where we collect the red fluorescence (>600 nm) for semiquantitative analysis. With this system, ROS production as well as ventricular pressure and ECG in isolated perfused rat hearts are monitored throughout the reperfusion of global ischemia. Ischemia would decrease myocardial ROS production, while reperfusion would immediately result in sustained ROS overproduction. Optical mapping would provide information regarding the spatial distribution and temporal evolution of myocardial ROS production, which would enhance knowledge of the role of free radicals in cardiovascular biology.

原文英語
文章編號021012
期刊Journal of Biomedical Optics
11
發行號2
DOIs
出版狀態已發佈 - 三月 2006
對外發佈

ASJC Scopus subject areas

  • 生物醫學工程
  • 生物材料
  • 電子、光磁材料
  • 原子與分子物理與光學
  • 放射學、核子醫學和影像學
  • 放射與超音波技術
  • 臨床生物化學

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