Online RP-HPLC-DPPH screening method for detection of radical-scavenging phytochemicals from flowers of Acacia confusa

Jyh Horng Wu, Chih Yu Huang, Yu Tang Tung, Shang Tzen Chang

研究成果: 雜誌貢獻文章同行評審

71 引文 斯高帕斯(Scopus)

摘要

Acacia confusa is traditionally used as a medicinal plant in Taiwan. In this study, phytochemicals and antioxidant activities of extracts from flowers of A. confusa were investigated for the first time. In addition, a rapid screening method, online RP-HPLC-DPPH system, for individual antioxidants in complex matrices was developed. Accordingly, six antioxidants including gallic acid (1), myricetin 3-rhamnoside (2), quercetin 3-rhamnoside (3), kaempferol 3-rhamnoside (4), europetin 3-rhamnoside (5), and rhamnetin 3-rhamnoside (6) were detected using the developed screening method. Of these, compounds 2, 3, and 5 were found to be major bioactive phytochemicals, and their contents were determined as 11.3, 6.7, and 8.7 mg/g of crude extract, respectively. By comparison with quercetin, a well-known antioxidant, these compounds had the order of compound 2 > compound 5 > quercetin > compound 3 for DPPH radical-scavenging activity. Their IC50 values were 3.0, 3.2, 4.5, and 7.4 μM, respectively. Moreover, the same order was observed for superoxide radical-scavenging activity, and their IC50 values were 2.6, 2.7, 4.3, and 5.3 μM, respectively. However, for lipid peroxidation, quercetin, an aglycon, showed the best inhibitory activity. The IC50 values of quercetin, compound 2, compound 5, and compound 3 were 46.7, 88.5, 90.7, and 124.6 μM, respectively. These results indicated that a rhamnoside at the C3 position of flavonoids had a negative effect on radical-scavenging activity and antilipid peroxidation. In contrast, the number of hydroxyl groups on the B-ring exhibited a positive relationship with their inhibitory activities.
原文英語
頁(從 - 到)328-332
頁數5
期刊Journal of Agricultural and Food Chemistry
56
發行號2
DOIs
出版狀態已發佈 - 一月 23 2008
對外發佈Yes

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Chemistry (miscellaneous)

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