Occurrence of D-amino acids and a pyridoxal 5′-phosphate-dependent aspartate racemase in the acidothermophilic archaeon, Thermoplasma acidophilum

Z. Long, J. A. Lee, T. Okamoto, M. Sekine, N. Nimura, K. Imai, M. Yohda, T. Maruyama, M. Sumi, N. Kamo, A. Yamagishi, T. Oshima, H. Homma

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28 引文 斯高帕斯(Scopus)

摘要

Free D-amino acid content in some archaea was investigated and D-forms of several amino acids were found in them. In the acidothermophilic archaeon, Thermoplasma acidophilum, the proportion of D-aspartate (D-Asp) to total Asp was as high as 39.7%. Crude extracts of Thermoplasma acidophilum had Asp-specific racemase activity that was pyridoxal 5′-phosphate (PLP)-dependent. The relative insensitivity to a SH-modifying reagent distinguished this activity from those of the PLP-independent Asp racemases found in other hyperthermophilic archaea (Matsumoto, M., et al., J. Bacteriol. 181, 6560-6563 1999). Thus, high levels of D-Asp should be produced by a new type(s) of Asp-specific racemase in Thermoplasma acidophilum, although the function of D-Asp in this archaeon remains unknown.
原文英語
頁(從 - 到)317-321
頁數5
期刊Biochemical and Biophysical Research Communications
281
發行號2
DOIs
出版狀態已發佈 - 2001

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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