Observation of the absorption of the In-DTPA-hexa-lactoside (IDHL) and its distribution in vitro by ICP-MS and TOF-SIMS

Chun-Chao Chang, Chun Nan Chen, Wan Lu Liao, Tsui Yun Lo, Shiou Ling Lei, Fu-Der Mai

研究成果: 雜誌貢獻文章同行評審

2 引文 斯高帕斯(Scopus)

摘要

The distribution of carbohydrate drug containing indiums 'In-DTPA-hexa-lactoside (IDHL)' in human hepatoblastoma cell line (HepG2) had been observed, and cell viability was investigated at the same time. The cell survival and mitochondrial metabolism of HepG2 after treated with 50 μΜ IDHL for 24 h were investigated by 3-[4,5-dimethylthiazol-2-yl]-2,5- diphenyltetra-zolium bromide assay, and the cytotoxicity was evaluated by lactate dehydrogenase method (LDH assay). The intracellular indium ion concentration was quantitated by inductively coupled plasma mass spectrometer. Besides, the distribution of indium ion in HepG2 was also analyzed by time-of-flight secondary ion mass spectrometry for comparison. The results demonstrated that there were only small amount of indium ions distributed inside the cytoplasm and the cell membrane. This means that HepG2 had very limited IDHL absorption, and there was almost no attachment of IDHL onto the HepG2 cell membrane. In these experiments, a parsing technique was established, which can shed some light on the understanding toward the absorption and distribution of these isotope compounds in vitro.
原文英語
頁(從 - 到)251-254
頁數4
期刊Surface and Interface Analysis
45
發行號1
DOIs
出版狀態已發佈 - 一月 2013

ASJC Scopus subject areas

  • 化學 (全部)
  • 凝聚態物理學
  • 表面和介面
  • 材料化學
  • 表面、塗料和薄膜

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