Nuclear translocation of cytochrome c during apoptosis

Alam Nur-E-Kamal, Stephane R. Gross, Zui Pan, Zita Balklava, Jianjie Ma, Leroy F. Liu

研究成果: 雜誌貢獻文章同行評審

102 引文 斯高帕斯(Scopus)


Release of cytochrome c from mitochondria is a major event during apoptosis. Released cytochrome c has been shown to activate caspase-dependent apoptotic signals. In this report, we provide evidence for a novel role of cytochrome c in caspase-independent nuclear apoptosis. We showed that cytochrome c, released from mitochondria upon apoptosis induction, gradually accumulates in the nucleus as evidenced by both immunofluorescence and subcellular fractionation. Parallel to nuclear accumulation of cytochrome c, acetylated histone H2A, but not unmodified H2A, was released from the nucleus to the cytoplasm. Addition of purified cytochrome c to isolated nuclei recapitulated the preferential release of acetylated, but not deacetylated, histone H2A. Cytochrome c was also found to induce chromatin condensation. These results suggest that the nuclear accumulation of cytochrome c may be directly involved in the remodeling of chromatin. Our results provide evidence of a novel role for cytochrome c in inducing nuclear apoptosis.

頁(從 - 到)24911-24914
期刊Journal of Biological Chemistry
出版狀態已發佈 - 6月 11 2004

ASJC Scopus subject areas

  • 生物化學
  • 分子生物學
  • 細胞生物學


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