Novel transmembrane GTPase of non-small cell lung cancer identified by mRNA differential display

J. G. Chung, K. T. Yeh, S. L. Wu, N. Y. Hsu, G. W. Chen, Y. W. Yeh, H. C. Ho

研究成果: 雜誌貢獻文章

45 引文 (Scopus)

摘要

The technique of differential display was used previously to profile the gene expression patterns of non-small cell lung cancer, and several genes differentially expressed were thus identified. In this report, we demonstrate that a DNA fragment of 347-bp length, up-regulated in tumor tissues, showed 100% sequence similarity to human cDNA FLJ20693 for a 370-residue protein. The gene product of cDNA FLJ20693 was postulated to be a shorter isoform of transmembrane GTPase, termed TG370, based upon the results of searching for sequence homology. The nucleotide sequence alignment also indicated that the cDNA FLJ20693 and the cDNA for 741-residue human mitofusin 1 (TG741) possibly resulted from the event of alternative splicing from which a 127-bp region was retained in the latter. Analysis of the genome sequence confirmed the speculation that both cDNAs were mapped to the same chromosomal position composing of 18 exons, of which the 127-bp region of TG741 constituted exon 11. The alternative splicing in all lung cancer cell lines was also observed to occur nearly in all tissue specimens examined. The up-regulated expression of transmembrane GTPase was subsequently found in tumor tissues from at least five of seven non-small cell lung cancer patients. Also, a distinct PCR product was initially detected in cell line H520, and further sequence analysis identified the presence of the 86-bp region mapped to the genome sequence immediately followed by exon 10. To evaluate the retention of 86-bp region, it was found that, besides the predicted 486-bp product, an unexpected 332-bp product was concomitantly observed and identified as the result of exon 8 deletion. The expression and subcellular localization of the full-length TG741 and other shorter isoforms were detected by flow cytometry using three polyclonal antibodies. It was concluded that the full-length TG741 located at plasma membrane with its NH2-terminal domain exposed extracellularly and the shorter isoforms retained at cytosol. Finally, the up-regulation of transmembrane GTPase in tumor tissues was further illustrated using immunohistochemical staining.
原文英語
頁(從 - 到)8873-8879
頁數7
期刊Cancer Research
61
發行號24
出版狀態已發佈 - 十二月 15 2001
對外發佈Yes

指紋

GTP Phosphohydrolases
Gene Expression Profiling
Non-Small Cell Lung Carcinoma
Complementary DNA
Exons
Protein Isoforms
Alternative Splicing
Sequence Analysis
Genome
Cell Line
Neoplasms
Sequence Alignment
Neoplasm Genes
Sequence Homology
Transcriptome
Cytosol
Lung Neoplasms
Flow Cytometry
Up-Regulation
Cell Membrane

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

引用此文

Chung, J. G., Yeh, K. T., Wu, S. L., Hsu, N. Y., Chen, G. W., Yeh, Y. W., & Ho, H. C. (2001). Novel transmembrane GTPase of non-small cell lung cancer identified by mRNA differential display. Cancer Research, 61(24), 8873-8879.

Novel transmembrane GTPase of non-small cell lung cancer identified by mRNA differential display. / Chung, J. G.; Yeh, K. T.; Wu, S. L.; Hsu, N. Y.; Chen, G. W.; Yeh, Y. W.; Ho, H. C.

於: Cancer Research, 卷 61, 編號 24, 15.12.2001, p. 8873-8879.

研究成果: 雜誌貢獻文章

Chung, JG, Yeh, KT, Wu, SL, Hsu, NY, Chen, GW, Yeh, YW & Ho, HC 2001, 'Novel transmembrane GTPase of non-small cell lung cancer identified by mRNA differential display', Cancer Research, 卷 61, 編號 24, 頁 8873-8879.
Chung JG, Yeh KT, Wu SL, Hsu NY, Chen GW, Yeh YW 等. Novel transmembrane GTPase of non-small cell lung cancer identified by mRNA differential display. Cancer Research. 2001 12月 15;61(24):8873-8879.
Chung, J. G. ; Yeh, K. T. ; Wu, S. L. ; Hsu, N. Y. ; Chen, G. W. ; Yeh, Y. W. ; Ho, H. C. / Novel transmembrane GTPase of non-small cell lung cancer identified by mRNA differential display. 於: Cancer Research. 2001 ; 卷 61, 編號 24. 頁 8873-8879.
@article{e43e893e11ab46ecba0b0d57a3475acc,
title = "Novel transmembrane GTPase of non-small cell lung cancer identified by mRNA differential display",
abstract = "The technique of differential display was used previously to profile the gene expression patterns of non-small cell lung cancer, and several genes differentially expressed were thus identified. In this report, we demonstrate that a DNA fragment of 347-bp length, up-regulated in tumor tissues, showed 100{\%} sequence similarity to human cDNA FLJ20693 for a 370-residue protein. The gene product of cDNA FLJ20693 was postulated to be a shorter isoform of transmembrane GTPase, termed TG370, based upon the results of searching for sequence homology. The nucleotide sequence alignment also indicated that the cDNA FLJ20693 and the cDNA for 741-residue human mitofusin 1 (TG741) possibly resulted from the event of alternative splicing from which a 127-bp region was retained in the latter. Analysis of the genome sequence confirmed the speculation that both cDNAs were mapped to the same chromosomal position composing of 18 exons, of which the 127-bp region of TG741 constituted exon 11. The alternative splicing in all lung cancer cell lines was also observed to occur nearly in all tissue specimens examined. The up-regulated expression of transmembrane GTPase was subsequently found in tumor tissues from at least five of seven non-small cell lung cancer patients. Also, a distinct PCR product was initially detected in cell line H520, and further sequence analysis identified the presence of the 86-bp region mapped to the genome sequence immediately followed by exon 10. To evaluate the retention of 86-bp region, it was found that, besides the predicted 486-bp product, an unexpected 332-bp product was concomitantly observed and identified as the result of exon 8 deletion. The expression and subcellular localization of the full-length TG741 and other shorter isoforms were detected by flow cytometry using three polyclonal antibodies. It was concluded that the full-length TG741 located at plasma membrane with its NH2-terminal domain exposed extracellularly and the shorter isoforms retained at cytosol. Finally, the up-regulation of transmembrane GTPase in tumor tissues was further illustrated using immunohistochemical staining.",
author = "Chung, {J. G.} and Yeh, {K. T.} and Wu, {S. L.} and Hsu, {N. Y.} and Chen, {G. W.} and Yeh, {Y. W.} and Ho, {H. C.}",
year = "2001",
month = "12",
day = "15",
language = "English",
volume = "61",
pages = "8873--8879",
journal = "Cancer Research",
issn = "0008-5472",
publisher = "American Association for Cancer Research Inc.",
number = "24",

}

TY - JOUR

T1 - Novel transmembrane GTPase of non-small cell lung cancer identified by mRNA differential display

AU - Chung, J. G.

AU - Yeh, K. T.

AU - Wu, S. L.

AU - Hsu, N. Y.

AU - Chen, G. W.

AU - Yeh, Y. W.

AU - Ho, H. C.

PY - 2001/12/15

Y1 - 2001/12/15

N2 - The technique of differential display was used previously to profile the gene expression patterns of non-small cell lung cancer, and several genes differentially expressed were thus identified. In this report, we demonstrate that a DNA fragment of 347-bp length, up-regulated in tumor tissues, showed 100% sequence similarity to human cDNA FLJ20693 for a 370-residue protein. The gene product of cDNA FLJ20693 was postulated to be a shorter isoform of transmembrane GTPase, termed TG370, based upon the results of searching for sequence homology. The nucleotide sequence alignment also indicated that the cDNA FLJ20693 and the cDNA for 741-residue human mitofusin 1 (TG741) possibly resulted from the event of alternative splicing from which a 127-bp region was retained in the latter. Analysis of the genome sequence confirmed the speculation that both cDNAs were mapped to the same chromosomal position composing of 18 exons, of which the 127-bp region of TG741 constituted exon 11. The alternative splicing in all lung cancer cell lines was also observed to occur nearly in all tissue specimens examined. The up-regulated expression of transmembrane GTPase was subsequently found in tumor tissues from at least five of seven non-small cell lung cancer patients. Also, a distinct PCR product was initially detected in cell line H520, and further sequence analysis identified the presence of the 86-bp region mapped to the genome sequence immediately followed by exon 10. To evaluate the retention of 86-bp region, it was found that, besides the predicted 486-bp product, an unexpected 332-bp product was concomitantly observed and identified as the result of exon 8 deletion. The expression and subcellular localization of the full-length TG741 and other shorter isoforms were detected by flow cytometry using three polyclonal antibodies. It was concluded that the full-length TG741 located at plasma membrane with its NH2-terminal domain exposed extracellularly and the shorter isoforms retained at cytosol. Finally, the up-regulation of transmembrane GTPase in tumor tissues was further illustrated using immunohistochemical staining.

AB - The technique of differential display was used previously to profile the gene expression patterns of non-small cell lung cancer, and several genes differentially expressed were thus identified. In this report, we demonstrate that a DNA fragment of 347-bp length, up-regulated in tumor tissues, showed 100% sequence similarity to human cDNA FLJ20693 for a 370-residue protein. The gene product of cDNA FLJ20693 was postulated to be a shorter isoform of transmembrane GTPase, termed TG370, based upon the results of searching for sequence homology. The nucleotide sequence alignment also indicated that the cDNA FLJ20693 and the cDNA for 741-residue human mitofusin 1 (TG741) possibly resulted from the event of alternative splicing from which a 127-bp region was retained in the latter. Analysis of the genome sequence confirmed the speculation that both cDNAs were mapped to the same chromosomal position composing of 18 exons, of which the 127-bp region of TG741 constituted exon 11. The alternative splicing in all lung cancer cell lines was also observed to occur nearly in all tissue specimens examined. The up-regulated expression of transmembrane GTPase was subsequently found in tumor tissues from at least five of seven non-small cell lung cancer patients. Also, a distinct PCR product was initially detected in cell line H520, and further sequence analysis identified the presence of the 86-bp region mapped to the genome sequence immediately followed by exon 10. To evaluate the retention of 86-bp region, it was found that, besides the predicted 486-bp product, an unexpected 332-bp product was concomitantly observed and identified as the result of exon 8 deletion. The expression and subcellular localization of the full-length TG741 and other shorter isoforms were detected by flow cytometry using three polyclonal antibodies. It was concluded that the full-length TG741 located at plasma membrane with its NH2-terminal domain exposed extracellularly and the shorter isoforms retained at cytosol. Finally, the up-regulation of transmembrane GTPase in tumor tissues was further illustrated using immunohistochemical staining.

UR - http://www.scopus.com/inward/record.url?scp=0035893566&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035893566&partnerID=8YFLogxK

M3 - Article

C2 - 11751411

AN - SCOPUS:0035893566

VL - 61

SP - 8873

EP - 8879

JO - Cancer Research

JF - Cancer Research

SN - 0008-5472

IS - 24

ER -