Nodal promotes growth and invasion in human gliomas

C. C. Lee, H. J. Jan, J. H. Lai, H. I. Ma, D. Y. Hueng, Y. C. Gladys Lee, Y. Y. Cheng, L. W. Liu, H. W. Wei, H. M. Lee

研究成果: 雜誌貢獻文章

80 引文 (Scopus)

摘要

Uncontrolled growth and diffused invasion are major causes of mortality in patients with malignant gliomas. Nodal has been shown to have a central role in the tumorigenic signaling pathways of malignant melanoma. In this study, we show that grade IV human glioma cell lines expressed different levels of Nodal, paralleled to the potential for cell invasiveness. Treatment of glioma cell lines with recombinant Nodal (rNodal) increased matrix metalloproteinase 2 (MMP-2) secretion and cell invasiveness. The ectopic expression of Nodal in GBM glioma cells that expressed Nodal at low level resulted in increased MMP-2 secretion, enhanced cell invasiveness, raised cell proliferation rates in vitro, increased tumor growth in vivo, and was associated with poor survival in a mice xenograft model. In contrast, the knockdown of Nodal expression in U87MG glioma cells with high Nodal expression level had reduced MMP-2 secretion, less cell invasiveness, lower tumor growth in vivo and longer lifespan in mice with U87MG/shNodal cell xenografts. In addition, Nodal knockdown promoted the reversion of malignant glioma cells toward a differentiated astrocytic phenotype. Furthermore, our data support the notion that Nodal may regulate glioma progression through the induction of the leukemia inhibitory factor (LIF) and Cripto-1 through activated Smad.
原文英語
頁(從 - 到)3110-3123
頁數14
期刊Oncogene
29
發行號21
DOIs
出版狀態已發佈 - 五月 27 2010

指紋

Glioma
Growth
Matrix Metalloproteinase 2
Heterografts
Leukemia Inhibitory Factor
Cell Line
Melanoma
Neoplasms
Cell Proliferation
Phenotype
Survival
Mortality

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

引用此文

Lee, C. C., Jan, H. J., Lai, J. H., Ma, H. I., Hueng, D. Y., Gladys Lee, Y. C., ... Lee, H. M. (2010). Nodal promotes growth and invasion in human gliomas. Oncogene, 29(21), 3110-3123. https://doi.org/10.1038/onc.2010.55

Nodal promotes growth and invasion in human gliomas. / Lee, C. C.; Jan, H. J.; Lai, J. H.; Ma, H. I.; Hueng, D. Y.; Gladys Lee, Y. C.; Cheng, Y. Y.; Liu, L. W.; Wei, H. W.; Lee, H. M.

於: Oncogene, 卷 29, 編號 21, 27.05.2010, p. 3110-3123.

研究成果: 雜誌貢獻文章

Lee, CC, Jan, HJ, Lai, JH, Ma, HI, Hueng, DY, Gladys Lee, YC, Cheng, YY, Liu, LW, Wei, HW & Lee, HM 2010, 'Nodal promotes growth and invasion in human gliomas', Oncogene, 卷 29, 編號 21, 頁 3110-3123. https://doi.org/10.1038/onc.2010.55
Lee CC, Jan HJ, Lai JH, Ma HI, Hueng DY, Gladys Lee YC 等. Nodal promotes growth and invasion in human gliomas. Oncogene. 2010 5月 27;29(21):3110-3123. https://doi.org/10.1038/onc.2010.55
Lee, C. C. ; Jan, H. J. ; Lai, J. H. ; Ma, H. I. ; Hueng, D. Y. ; Gladys Lee, Y. C. ; Cheng, Y. Y. ; Liu, L. W. ; Wei, H. W. ; Lee, H. M. / Nodal promotes growth and invasion in human gliomas. 於: Oncogene. 2010 ; 卷 29, 編號 21. 頁 3110-3123.
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