Nitric oxide synthesis is increased in the endometrial tissue of women with endometriosis

Ming Yih Wu, Kuang Han Chao, Jehn Hsiahn Yang, Tsung Hsien Lee, Yu Shih Yang, Hong Nerng Ho

研究成果: 雜誌貢獻文章

49 引文 (Scopus)

摘要

Background: Previous studies have shown that peritoneal macrophages from women with endometriosis produce excess nitric oxide (NO). This study was designed to quantify the amount of NO and determine the expression of endothelial (eNOS) and inducible NO synthases (iNOS) in women with and without endometriosis. Methods: An enzyme-linked immunosorbent assay (ELISA) was performed on endometrial tissues obtained from controls (myoma, n = 30) and on eutopic/ectopic endometrial tissues from endometriosis patients (n = 34) to evaluate eNOS and iNOS protein concentrations in these endometrial tissues. A rapid-response chemiluminescence analyser was used to measure NO directly in fresh endometrial tissues. Results: Mean (± SEM) levels of NO were significantly increased in the endometrial tissues of women with endometriosis (13.2 ± 7.8 versus 19.8 ± 12.6 nmol/g tissue; P = 0.016). Apparently higher levels of NO were found in ectopic compared with eutopic endometrium (P = 0.057). Endometrial tissues of women with endometriosis appeared to contain more iNOS than those of controls (3.6 ± 2.2 versus 8.6 ± 12.2 pg/μg protein; P = 0.06), but no significant difference was found in eNOS levels. Conclusions: Greater amounts of NO and NOS are present in the endometrial tissues of women with endometriosis, implying a possible role for NO in the pathogenesis of endometriosis.
原文英語
頁(從 - 到)2668-2671
頁數4
期刊Human Reproduction
18
發行號12
DOIs
出版狀態已發佈 - 一月 1 2003
對外發佈Yes

指紋

Luminescent Measurements
Nitric Oxide Synthase Type III
Endometriosis
Nitrites
Endometrium
Nitric Oxide Synthase
Nitrates
Nitric Oxide
Enzyme-Linked Immunosorbent Assay
Immunohistochemistry
Choristoma
Myoma
Peritoneal Macrophages
Nitric Oxide Synthase Type II
Luminescence
Proteins

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynaecology

引用此文

Nitric oxide synthesis is increased in the endometrial tissue of women with endometriosis. / Wu, Ming Yih; Chao, Kuang Han; Yang, Jehn Hsiahn; Lee, Tsung Hsien; Yang, Yu Shih; Ho, Hong Nerng.

於: Human Reproduction, 卷 18, 編號 12, 01.01.2003, p. 2668-2671.

研究成果: 雜誌貢獻文章

Wu, Ming Yih ; Chao, Kuang Han ; Yang, Jehn Hsiahn ; Lee, Tsung Hsien ; Yang, Yu Shih ; Ho, Hong Nerng. / Nitric oxide synthesis is increased in the endometrial tissue of women with endometriosis. 於: Human Reproduction. 2003 ; 卷 18, 編號 12. 頁 2668-2671.
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abstract = "Background: Previous studies have shown that peritoneal macrophages from women with endometriosis produce excess nitric oxide (NO). This study was designed to quantify the amount of NO and determine the expression of endothelial (eNOS) and inducible NO synthases (iNOS) in women with and without endometriosis. Methods: An enzyme-linked immunosorbent assay (ELISA) was performed on endometrial tissues obtained from controls (myoma, n = 30) and on eutopic/ectopic endometrial tissues from endometriosis patients (n = 34) to evaluate eNOS and iNOS protein concentrations in these endometrial tissues. A rapid-response chemiluminescence analyser was used to measure NO directly in fresh endometrial tissues. Results: Mean (± SEM) levels of NO were significantly increased in the endometrial tissues of women with endometriosis (13.2 ± 7.8 versus 19.8 ± 12.6 nmol/g tissue; P = 0.016). Apparently higher levels of NO were found in ectopic compared with eutopic endometrium (P = 0.057). Endometrial tissues of women with endometriosis appeared to contain more iNOS than those of controls (3.6 ± 2.2 versus 8.6 ± 12.2 pg/μg protein; P = 0.06), but no significant difference was found in eNOS levels. Conclusions: Greater amounts of NO and NOS are present in the endometrial tissues of women with endometriosis, implying a possible role for NO in the pathogenesis of endometriosis.",
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AU - Chao, Kuang Han

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AU - Yang, Yu Shih

AU - Ho, Hong Nerng

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N2 - Background: Previous studies have shown that peritoneal macrophages from women with endometriosis produce excess nitric oxide (NO). This study was designed to quantify the amount of NO and determine the expression of endothelial (eNOS) and inducible NO synthases (iNOS) in women with and without endometriosis. Methods: An enzyme-linked immunosorbent assay (ELISA) was performed on endometrial tissues obtained from controls (myoma, n = 30) and on eutopic/ectopic endometrial tissues from endometriosis patients (n = 34) to evaluate eNOS and iNOS protein concentrations in these endometrial tissues. A rapid-response chemiluminescence analyser was used to measure NO directly in fresh endometrial tissues. Results: Mean (± SEM) levels of NO were significantly increased in the endometrial tissues of women with endometriosis (13.2 ± 7.8 versus 19.8 ± 12.6 nmol/g tissue; P = 0.016). Apparently higher levels of NO were found in ectopic compared with eutopic endometrium (P = 0.057). Endometrial tissues of women with endometriosis appeared to contain more iNOS than those of controls (3.6 ± 2.2 versus 8.6 ± 12.2 pg/μg protein; P = 0.06), but no significant difference was found in eNOS levels. Conclusions: Greater amounts of NO and NOS are present in the endometrial tissues of women with endometriosis, implying a possible role for NO in the pathogenesis of endometriosis.

AB - Background: Previous studies have shown that peritoneal macrophages from women with endometriosis produce excess nitric oxide (NO). This study was designed to quantify the amount of NO and determine the expression of endothelial (eNOS) and inducible NO synthases (iNOS) in women with and without endometriosis. Methods: An enzyme-linked immunosorbent assay (ELISA) was performed on endometrial tissues obtained from controls (myoma, n = 30) and on eutopic/ectopic endometrial tissues from endometriosis patients (n = 34) to evaluate eNOS and iNOS protein concentrations in these endometrial tissues. A rapid-response chemiluminescence analyser was used to measure NO directly in fresh endometrial tissues. Results: Mean (± SEM) levels of NO were significantly increased in the endometrial tissues of women with endometriosis (13.2 ± 7.8 versus 19.8 ± 12.6 nmol/g tissue; P = 0.016). Apparently higher levels of NO were found in ectopic compared with eutopic endometrium (P = 0.057). Endometrial tissues of women with endometriosis appeared to contain more iNOS than those of controls (3.6 ± 2.2 versus 8.6 ± 12.2 pg/μg protein; P = 0.06), but no significant difference was found in eNOS levels. Conclusions: Greater amounts of NO and NOS are present in the endometrial tissues of women with endometriosis, implying a possible role for NO in the pathogenesis of endometriosis.

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