Nitric oxide produced by iNOS is associated with collagen synthesis in keloid scar formation

Yi Chiang Hsu, Michael Hsiao, Leng-Fang Wang, Yie W. Chien, Woan Ruoh Lee

研究成果: 雜誌貢獻文章同行評審

45 引文 斯高帕斯(Scopus)

摘要

Nitric oxide (NO) has emerged as an important mediator of many physiological functions. Recent reports have shown that NO participates in the wound healing process, however, its role in keloid formation remains unclear. This study aimed to investigate the effect of NO on keloid fibroblasts (KF) and to determine the levels of inducible nitric oxide synthase (iNOS) expression in clinical specimens of keloid. Scar tissue from seven keloid patients with matched perilesion skin tissue controls was studied for inducible nitric oxide synthase expression and location. In addition, primary keloid and normal scar skin fibroblast cultures were set up to investigate the effects of NO in inducing collagen type I expression. Inducible nitric oxide synthase expression, and NO production were elevated in keloid scar tissues but not in matched perilesion skin tissues. Furthermore, exposure of KF to exogenous NO resulted in increased expression of collagen type I in a dose-dependent manner. NO exposure also induced time-course dependent collagen I expression that peaked at 24 h in KF. Taken together, these results indicate that excess collagen formations in keloid lesion may be attributed to iNOS overexpression.
原文英語
頁(從 - 到)327-334
頁數8
期刊Nitric Oxide - Biology and Chemistry
14
發行號4
DOIs
出版狀態已發佈 - 6月 2006

ASJC Scopus subject areas

  • 生物化學
  • 分子生物學

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