New constituents with iNOS inhibitory activity from mycelium of Antrodia camphorata

Sien Sing Yang, Guei Jane Wang, Shiang Yi Wang, Yu Ying Lin, Yueh Hsiung Kuo, Zong-Huei Li

研究成果: 雜誌貢獻文章同行評審

51 引文 斯高帕斯(Scopus)

摘要

In continuing our investigation on the bioactive constituents ofmycelium of Antrodia camphorata, antroquinonol B (1), 4-acetyl-antroquinonol B (2), 2,3-(methylenedioxy)-6-methylbenzene-1,4-diol (3) and 2,4-dimethoxy-6- methylbenzene-1,3-diol (4) along with antrodin D (5) were isolated by the guidance of an inducible nitric oxide synthase (iNOS) inhibitory assay and identified on the basis of their spectroscopic analysis. The effect of these compounds on the inhibition of NO production in lipopolysaccharide (LPS)-activated murine macrophages was further evaluated. Compounds 4 and 5 significantly inhibited NO production without any cytotoxicity, the IC 50 values being 32.2 ± 0.1 and 26.3 ± 1.6 μ g/mL, respectively. Compounds 1 and 2 possessed greater effects on NO inhibition, with IC50 values of 16.2 ± 0.8 and 14.7 ± 2.8 μ g/mL, respectively, but displayed cytotoxicity at considerably higher concentrations. Compound 3 showed the lowest percent cell viability of 45.5 ± 1.8% as observed in treated cells at a concentration of 16.8 μ g/mL.
原文英語
頁(從 - 到)512-516
頁數5
期刊Planta Medica
75
發行號5
DOIs
出版狀態已發佈 - 4月 2009

ASJC Scopus subject areas

  • 補充和替代醫學
  • 分子醫學
  • 有機化學
  • 分析化學
  • 藥學科學
  • 藥理
  • 藥物發現

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