New constituent from Podocarpus macrophyllus var. macrophyllus shows anti-tyrosinase effect and regulates tyrosinase-related proteins and mRNA in human epidermal melanocytes

Kur Ta Cheng, Feng Lin Hsu, Shih Hui Chen, Peng Ke Hsieh, Hsu Shan Huang, Ching Kuo Lee, Mei Hsien Lee

研究成果: 雜誌貢獻文章同行評審

21 引文 斯高帕斯(Scopus)

摘要

A new biflavonoid, 2,3-dihydro-4′,4‴-di-O-methylamentoflavone (5), and five known compounds, (-)-catechin (1), quercetin (2), 2,3-dihydrosciadopitysin (3), sciadopitysin (4), and isoginkgetin (6), were isolated from Podocarpus macrophyllus var. macrophyllus (Podocarpaceae). These compounds were evaluated their ability to inhibit cellular tyrosinase activity and for their melanin inhibitory activity in human epidermal melanocytes (HEMn). In the melanin synthesis assay, 2,3-dihydro-4′,4‴-di-O- methylamentoflavone (5) showed a potent anti-tyrosinase effect with IC 50=0.098 mM in HEMn. It also significantly decreased both protein and mRNA levels of the tyrosinase-related protein-2 (TRP-2) by Western blot and quantitative real-time PCR (qRT-PCR) analysis. These findings suggest that the new compound, 2,3-dihydro-4′,4‴-di-O-methylamentoflavone (5), is the most active component of P. macrophyllus var. macrophyllus in inhibiting pigmentation and that this inhibition is exerted through inhibition of transcription of the genes encoding TRP2.
原文英語
頁(從 - 到)757-761
頁數5
期刊Chemical and Pharmaceutical Bulletin
55
發行號5
DOIs
出版狀態已發佈 - 五月 2007

ASJC Scopus subject areas

  • 藥物發現
  • 有機化學
  • 化學 (全部)
  • 藥理

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