Neuroprotective effect of agmatine in rats with transient cerebral ischemia using MR imaging and histopathologic evaluation

Y. C. Huang, W. S. Tzeng, C. C. Wang, B. C. Cheng, Y. K. Chang, H. H. Chen, P. C. Lin, T. Y. Huang, T. J. Chuang, J. W. Lin, C. P. Chang

研究成果: 雜誌貢獻文章

12 引文 (Scopus)

摘要

Purpose: This study aimed to further investigate the effects of agmatine on brain edema in the rats with middle cerebral artery occlusion (MCAO) injury using magnetic resonance imaging (MRI) monitoring and biochemical and histopathologic evaluation. Materials and methods: Following surgical induction of MCAO for 90. min, agmatine was injected 5. min after beginning of reperfusion and again once daily for the next 3 post-operative days. The events during ischemia and reperfusion were investigated by T2-weighted images (T2WI), serial diffusion-weighted images (DWI), calculated apparent diffusion coefficient (ADC) maps and contrast-enhanced T1-weighted images (CE-T1WI) during 3. h-72. h in a 1.5. T Siemens MAGNETON Avanto Scanner. Lesion volumes were analyzed in a blinded and randomized manner. Triphenyltetrazolium chloride (TTC), Nissl, and Evans Blue stainings were performed at the corresponding sections. Results: Increased lesion volumes derived from T2WI, DWI, ADC, CE-T1WI, and TTC all were noted at 3. h and peaked at 24. h-48. h after MCAO injury. TTC-derived infarct volumes were not significantly different from the T2WI, DWI-, and CE-T1WI-derived lesion volumes at the last imaging time (72. h) point except for significantly smaller ADC lesions in the MCAO model (P.
原文英語
頁(從 - 到)1174-1181
頁數8
期刊Magnetic Resonance Imaging
31
發行號7
DOIs
出版狀態已發佈 - 九月 2013

指紋

Agmatine
Transient Ischemic Attack
Neuroprotective Agents
Rats
Middle Cerebral Artery Infarction
Imaging techniques
Reperfusion
Evans Blue
Brain Edema
Wounds and Injuries
Magnetic resonance
Brain
Ischemia
Magnetic Resonance Imaging
Staining and Labeling
Monitoring
triphenyltetrazolium

ASJC Scopus subject areas

  • Biophysics
  • Radiology Nuclear Medicine and imaging
  • Biomedical Engineering

引用此文

Huang, Y. C., Tzeng, W. S., Wang, C. C., Cheng, B. C., Chang, Y. K., Chen, H. H., ... Chang, C. P. (2013). Neuroprotective effect of agmatine in rats with transient cerebral ischemia using MR imaging and histopathologic evaluation. Magnetic Resonance Imaging, 31(7), 1174-1181. https://doi.org/10.1016/j.mri.2013.03.026

Neuroprotective effect of agmatine in rats with transient cerebral ischemia using MR imaging and histopathologic evaluation. / Huang, Y. C.; Tzeng, W. S.; Wang, C. C.; Cheng, B. C.; Chang, Y. K.; Chen, H. H.; Lin, P. C.; Huang, T. Y.; Chuang, T. J.; Lin, J. W.; Chang, C. P.

於: Magnetic Resonance Imaging, 卷 31, 編號 7, 09.2013, p. 1174-1181.

研究成果: 雜誌貢獻文章

Huang, YC, Tzeng, WS, Wang, CC, Cheng, BC, Chang, YK, Chen, HH, Lin, PC, Huang, TY, Chuang, TJ, Lin, JW & Chang, CP 2013, 'Neuroprotective effect of agmatine in rats with transient cerebral ischemia using MR imaging and histopathologic evaluation', Magnetic Resonance Imaging, 卷 31, 編號 7, 頁 1174-1181. https://doi.org/10.1016/j.mri.2013.03.026
Huang, Y. C. ; Tzeng, W. S. ; Wang, C. C. ; Cheng, B. C. ; Chang, Y. K. ; Chen, H. H. ; Lin, P. C. ; Huang, T. Y. ; Chuang, T. J. ; Lin, J. W. ; Chang, C. P. / Neuroprotective effect of agmatine in rats with transient cerebral ischemia using MR imaging and histopathologic evaluation. 於: Magnetic Resonance Imaging. 2013 ; 卷 31, 編號 7. 頁 1174-1181.
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abstract = "Purpose: This study aimed to further investigate the effects of agmatine on brain edema in the rats with middle cerebral artery occlusion (MCAO) injury using magnetic resonance imaging (MRI) monitoring and biochemical and histopathologic evaluation. Materials and methods: Following surgical induction of MCAO for 90. min, agmatine was injected 5. min after beginning of reperfusion and again once daily for the next 3 post-operative days. The events during ischemia and reperfusion were investigated by T2-weighted images (T2WI), serial diffusion-weighted images (DWI), calculated apparent diffusion coefficient (ADC) maps and contrast-enhanced T1-weighted images (CE-T1WI) during 3. h-72. h in a 1.5. T Siemens MAGNETON Avanto Scanner. Lesion volumes were analyzed in a blinded and randomized manner. Triphenyltetrazolium chloride (TTC), Nissl, and Evans Blue stainings were performed at the corresponding sections. Results: Increased lesion volumes derived from T2WI, DWI, ADC, CE-T1WI, and TTC all were noted at 3. h and peaked at 24. h-48. h after MCAO injury. TTC-derived infarct volumes were not significantly different from the T2WI, DWI-, and CE-T1WI-derived lesion volumes at the last imaging time (72. h) point except for significantly smaller ADC lesions in the MCAO model (P.",
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AU - Huang, Y. C.

AU - Tzeng, W. S.

AU - Wang, C. C.

AU - Cheng, B. C.

AU - Chang, Y. K.

AU - Chen, H. H.

AU - Lin, P. C.

AU - Huang, T. Y.

AU - Chuang, T. J.

AU - Lin, J. W.

AU - Chang, C. P.

PY - 2013/9

Y1 - 2013/9

N2 - Purpose: This study aimed to further investigate the effects of agmatine on brain edema in the rats with middle cerebral artery occlusion (MCAO) injury using magnetic resonance imaging (MRI) monitoring and biochemical and histopathologic evaluation. Materials and methods: Following surgical induction of MCAO for 90. min, agmatine was injected 5. min after beginning of reperfusion and again once daily for the next 3 post-operative days. The events during ischemia and reperfusion were investigated by T2-weighted images (T2WI), serial diffusion-weighted images (DWI), calculated apparent diffusion coefficient (ADC) maps and contrast-enhanced T1-weighted images (CE-T1WI) during 3. h-72. h in a 1.5. T Siemens MAGNETON Avanto Scanner. Lesion volumes were analyzed in a blinded and randomized manner. Triphenyltetrazolium chloride (TTC), Nissl, and Evans Blue stainings were performed at the corresponding sections. Results: Increased lesion volumes derived from T2WI, DWI, ADC, CE-T1WI, and TTC all were noted at 3. h and peaked at 24. h-48. h after MCAO injury. TTC-derived infarct volumes were not significantly different from the T2WI, DWI-, and CE-T1WI-derived lesion volumes at the last imaging time (72. h) point except for significantly smaller ADC lesions in the MCAO model (P.

AB - Purpose: This study aimed to further investigate the effects of agmatine on brain edema in the rats with middle cerebral artery occlusion (MCAO) injury using magnetic resonance imaging (MRI) monitoring and biochemical and histopathologic evaluation. Materials and methods: Following surgical induction of MCAO for 90. min, agmatine was injected 5. min after beginning of reperfusion and again once daily for the next 3 post-operative days. The events during ischemia and reperfusion were investigated by T2-weighted images (T2WI), serial diffusion-weighted images (DWI), calculated apparent diffusion coefficient (ADC) maps and contrast-enhanced T1-weighted images (CE-T1WI) during 3. h-72. h in a 1.5. T Siemens MAGNETON Avanto Scanner. Lesion volumes were analyzed in a blinded and randomized manner. Triphenyltetrazolium chloride (TTC), Nissl, and Evans Blue stainings were performed at the corresponding sections. Results: Increased lesion volumes derived from T2WI, DWI, ADC, CE-T1WI, and TTC all were noted at 3. h and peaked at 24. h-48. h after MCAO injury. TTC-derived infarct volumes were not significantly different from the T2WI, DWI-, and CE-T1WI-derived lesion volumes at the last imaging time (72. h) point except for significantly smaller ADC lesions in the MCAO model (P.

KW - Agmatine

KW - Brain edema

KW - Magnetic resonance image

KW - Pharmacology

KW - Stroke

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