Neuroprotective effect of agmatine in rats with transient cerebral ischemia using MR imaging and histopathologic evaluation

Y. C. Huang; W. S. Tzeng; C. C. Wang; B. C. Cheng; Y. K. Chang; H. H. Chen; P. C. Lin; T. Y. Huang; T. J. Chuang; J. W. Lin; C. P. Chang

doi:10.1016/j.mri.2013.03.026

Neuroprotective effect of agmatine in rats with transient cerebral ischemia using MR imaging and histopathologic evaluation

Y. C. Huang, W. S. Tzeng, C. C. Wang, B. C. Cheng, Y. K. Chang, H. H. Chen, P. C. Lin, T. Y. Huang, T. J. Chuang, J. W. Lin, C. P. Chang

研究成果: 雜誌貢獻 › 文章 › 同行評審

15 引文斯高帕斯（Scopus）

摘要

Purpose: This study aimed to further investigate the effects of agmatine on brain edema in the rats with middle cerebral artery occlusion (MCAO) injury using magnetic resonance imaging (MRI) monitoring and biochemical and histopathologic evaluation. Materials and methods: Following surgical induction of MCAO for 90. min, agmatine was injected 5. min after beginning of reperfusion and again once daily for the next 3 post-operative days. The events during ischemia and reperfusion were investigated by T2-weighted images (T2WI), serial diffusion-weighted images (DWI), calculated apparent diffusion coefficient (ADC) maps and contrast-enhanced T1-weighted images (CE-T1WI) during 3. h-72. h in a 1.5. T Siemens MAGNETON Avanto Scanner. Lesion volumes were analyzed in a blinded and randomized manner. Triphenyltetrazolium chloride (TTC), Nissl, and Evans Blue stainings were performed at the corresponding sections. Results: Increased lesion volumes derived from T2WI, DWI, ADC, CE-T1WI, and TTC all were noted at 3. h and peaked at 24. h-48. h after MCAO injury. TTC-derived infarct volumes were not significantly different from the T2WI, DWI-, and CE-T1WI-derived lesion volumes at the last imaging time (72. h) point except for significantly smaller ADC lesions in the MCAO model (P.

原文	英語
頁（從 - 到）	1174-1181
頁數	8
期刊	Magnetic Resonance Imaging
卷	31
發行號	7
DOIs	https://doi.org/10.1016/j.mri.2013.03.026
出版狀態	已發佈 - 九月 2013

ASJC Scopus subject areas

Biophysics
Radiology Nuclear Medicine and imaging
Biomedical Engineering

存取文件

10.1016/j.mri.2013.03.026

指紋深入研究「Neuroprotective effect of agmatine in rats with transient cerebral ischemia using MR imaging and histopathologic evaluation」主題。共同形成了獨特的指紋。

引用此

Huang, Y. C., Tzeng, W. S., Wang, C. C., Cheng, B. C., Chang, Y. K., Chen, H. H., Lin, P. C., Huang, T. Y., Chuang, T. J., Lin, J. W., & Chang, C. P. (2013). Neuroprotective effect of agmatine in rats with transient cerebral ischemia using MR imaging and histopathologic evaluation. Magnetic Resonance Imaging, 31(7), 1174-1181. https://doi.org/10.1016/j.mri.2013.03.026

Neuroprotective effect of agmatine in rats with transient cerebral ischemia using MR imaging and histopathologic evaluation. / Huang, Y. C.; Tzeng, W. S.; Wang, C. C.; Cheng, B. C.; Chang, Y. K.; Chen, H. H.; Lin, P. C.; Huang, T. Y.; Chuang, T. J.; Lin, J. W.; Chang, C. P.

於: Magnetic Resonance Imaging, 卷 31, 編號 7, 09.2013, p. 1174-1181.

研究成果: 雜誌貢獻 › 文章 › 同行評審

@article{c2717ea8598b4dc989052f739c945ba9,

title = "Neuroprotective effect of agmatine in rats with transient cerebral ischemia using MR imaging and histopathologic evaluation",

abstract = "Purpose: This study aimed to further investigate the effects of agmatine on brain edema in the rats with middle cerebral artery occlusion (MCAO) injury using magnetic resonance imaging (MRI) monitoring and biochemical and histopathologic evaluation. Materials and methods: Following surgical induction of MCAO for 90. min, agmatine was injected 5. min after beginning of reperfusion and again once daily for the next 3 post-operative days. The events during ischemia and reperfusion were investigated by T2-weighted images (T2WI), serial diffusion-weighted images (DWI), calculated apparent diffusion coefficient (ADC) maps and contrast-enhanced T1-weighted images (CE-T1WI) during 3. h-72. h in a 1.5. T Siemens MAGNETON Avanto Scanner. Lesion volumes were analyzed in a blinded and randomized manner. Triphenyltetrazolium chloride (TTC), Nissl, and Evans Blue stainings were performed at the corresponding sections. Results: Increased lesion volumes derived from T2WI, DWI, ADC, CE-T1WI, and TTC all were noted at 3. h and peaked at 24. h-48. h after MCAO injury. TTC-derived infarct volumes were not significantly different from the T2WI, DWI-, and CE-T1WI-derived lesion volumes at the last imaging time (72. h) point except for significantly smaller ADC lesions in the MCAO model (P. ",

keywords = "Agmatine, Brain edema, Magnetic resonance image, Pharmacology, Stroke",

author = "Huang, {Y. C.} and Tzeng, {W. S.} and Wang, {C. C.} and Cheng, {B. C.} and Chang, {Y. K.} and Chen, {H. H.} and Lin, {P. C.} and Huang, {T. Y.} and Chuang, {T. J.} and Lin, {J. W.} and Chang, {C. P.}",

year = "2013",

month = sep,

doi = "10.1016/j.mri.2013.03.026",

language = "English",

volume = "31",

pages = "1174--1181",

journal = "Magnetic Resonance Imaging",

issn = "0730-725X",

publisher = "Elsevier Inc.",

number = "7",

}

TY - JOUR

T1 - Neuroprotective effect of agmatine in rats with transient cerebral ischemia using MR imaging and histopathologic evaluation

AU - Huang, Y. C.

AU - Tzeng, W. S.

AU - Wang, C. C.

AU - Cheng, B. C.

AU - Chang, Y. K.

AU - Chen, H. H.

AU - Lin, P. C.

AU - Huang, T. Y.

AU - Chuang, T. J.

AU - Lin, J. W.

AU - Chang, C. P.

PY - 2013/9

Y1 - 2013/9

N2 - Purpose: This study aimed to further investigate the effects of agmatine on brain edema in the rats with middle cerebral artery occlusion (MCAO) injury using magnetic resonance imaging (MRI) monitoring and biochemical and histopathologic evaluation. Materials and methods: Following surgical induction of MCAO for 90. min, agmatine was injected 5. min after beginning of reperfusion and again once daily for the next 3 post-operative days. The events during ischemia and reperfusion were investigated by T2-weighted images (T2WI), serial diffusion-weighted images (DWI), calculated apparent diffusion coefficient (ADC) maps and contrast-enhanced T1-weighted images (CE-T1WI) during 3. h-72. h in a 1.5. T Siemens MAGNETON Avanto Scanner. Lesion volumes were analyzed in a blinded and randomized manner. Triphenyltetrazolium chloride (TTC), Nissl, and Evans Blue stainings were performed at the corresponding sections. Results: Increased lesion volumes derived from T2WI, DWI, ADC, CE-T1WI, and TTC all were noted at 3. h and peaked at 24. h-48. h after MCAO injury. TTC-derived infarct volumes were not significantly different from the T2WI, DWI-, and CE-T1WI-derived lesion volumes at the last imaging time (72. h) point except for significantly smaller ADC lesions in the MCAO model (P.

AB - Purpose: This study aimed to further investigate the effects of agmatine on brain edema in the rats with middle cerebral artery occlusion (MCAO) injury using magnetic resonance imaging (MRI) monitoring and biochemical and histopathologic evaluation. Materials and methods: Following surgical induction of MCAO for 90. min, agmatine was injected 5. min after beginning of reperfusion and again once daily for the next 3 post-operative days. The events during ischemia and reperfusion were investigated by T2-weighted images (T2WI), serial diffusion-weighted images (DWI), calculated apparent diffusion coefficient (ADC) maps and contrast-enhanced T1-weighted images (CE-T1WI) during 3. h-72. h in a 1.5. T Siemens MAGNETON Avanto Scanner. Lesion volumes were analyzed in a blinded and randomized manner. Triphenyltetrazolium chloride (TTC), Nissl, and Evans Blue stainings were performed at the corresponding sections. Results: Increased lesion volumes derived from T2WI, DWI, ADC, CE-T1WI, and TTC all were noted at 3. h and peaked at 24. h-48. h after MCAO injury. TTC-derived infarct volumes were not significantly different from the T2WI, DWI-, and CE-T1WI-derived lesion volumes at the last imaging time (72. h) point except for significantly smaller ADC lesions in the MCAO model (P.

KW - Agmatine

KW - Brain edema

KW - Magnetic resonance image

KW - Pharmacology

KW - Stroke

UR - http://www.scopus.com/inward/record.url?scp=84880731514&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84880731514&partnerID=8YFLogxK

U2 - 10.1016/j.mri.2013.03.026

DO - 10.1016/j.mri.2013.03.026

M3 - Article

C2 - 23642800

AN - SCOPUS:84880731514

VL - 31

SP - 1174

EP - 1181

JO - Magnetic Resonance Imaging

JF - Magnetic Resonance Imaging

SN - 0730-725X

IS - 7

ER -