Neuropathic Allodynia Involves Spinal Neurexin-1β-dependent Neuroligin-1/Postsynaptic Density-95/NR2B Cascade in Rats

Tzer Bin Lin, Cheng Yuan Lai, Ming Chun Hsieh, Jian Lin Jiang, Jen Kun Cheng, Yat Pang Chau, Ting Ruan, Gin Den Chen, Hsien Yu Peng

研究成果: 雜誌貢獻文章

11 引文 斯高帕斯(Scopus)

摘要

Background: Neuroligin-1 (NL1) forms a complex with the presynaptic neurexin-1 (Nrx1b), regulating clustering of N-methyl-d-Aspartate receptors with postsynaptic density-95 (PSD-95) to underlie learning-/memory-Associated plasticity. Pain-related spinal neuroplasticity shares several common features with learning-/memory-Associated plasticity. The authors thereby investigated the potential involvement of NL1-related mechanism in spinal nerve ligation (SNL)-Associated allodynia. Methods: In 626 adult male Sprague-Dawley rats, the withdrawal threshold and NL1, PSD-95, phosphorylated NR2B (pNR2B) expressions, interactions, and locations in dorsal horn (L4 to L5) were compared between the sham operation and SNL groups. A recombinant Nrx1b Fc chimera (Nrx1b Fc, 10 μg, 10 μl, i.t., bolus), antisense small-interfering RNA targeting to NL1 (10 μg, 10 μl, i.t., daily for 4 days), or NR2B antagonist (Ro 25-6981; 1 μM, 10 μl, i.t., bolus) were administered to SNL animals to elucidate possible cascades involved. Results: SNL-induced allodynia failed to affect NL1 or PSD-95 expression. However, pNR2B expression (mean ± SD from 13.1 ± 2.87 to 23.1 ± 2.52, n = 6) and coexpression of NL1-PSD-95, pNR2B-PSD-95, and NL1-total NR2B were enhanced by SNL (from 10.7 ± 2.27 to 22.2 ± 3.94, 11.5 ± 2.15 to 23.8 ± 3.32, and 8.9 ± 1.83 to 14.9 ± 2.27 at day 7, n = 6). Furthermore, neuron-localized pNR2B PSD-95-pNR2B double-labeled and NL1/PSD-95/pNR2B triple-labeled immunofluorescence in the ipsilateral dorsal horn was all prevented by Nrx1b Fc and NL1-targeted small-interfering RNA designed to block and prevent NL1 expression. Without affecting NL1-PSD-95 coupling, Ro 25-6981 decreased the SNL-induced PSD-95-pNR2B coprecipitation (from 18.7 ± 1.80 to 14.7 ± 2.36 at day 7, n = 6). Conclusion: SNL-induced allodynia, which is mediated by the spinal NL1/PSD-95/pNR2B cascade, can be prevented by blockade of transsynaptic Nrx1b-NL1 interactions.

原文英語
頁(從 - 到)909-926
頁數18
期刊Anesthesiology
123
發行號4
出版狀態已發佈 - 十月 1 2015

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine

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    Lin, T. B., Lai, C. Y., Hsieh, M. C., Jiang, J. L., Cheng, J. K., Chau, Y. P., Ruan, T., Chen, G. D., & Peng, H. Y. (2015). Neuropathic Allodynia Involves Spinal Neurexin-1β-dependent Neuroligin-1/Postsynaptic Density-95/NR2B Cascade in Rats. Anesthesiology, 123(4), 909-926.