Netrin1 Produced by Neural Progenitors, Not Floor Plate Cells, Is Required for Axon Guidance in the Spinal Cord

Supraja G. Varadarajan, Jennifer H. Kong, Keith D. Phan, Tzu Jen Kao, S. Carmen Panaitof, Julie Cardin, Holger Eltzschig, Artur Kania, Bennett G. Novitch, Samantha J. Butler

研究成果: 雜誌貢獻文章

53 引文 (Scopus)

摘要

Netrin1 has been proposed to act from the floor plate (FP) as a long-range diffusible chemoattractant for commissural axons in the embryonic spinal cord. However, netrin1 mRNA and protein are also present in neural progenitors within the ventricular zone (VZ), raising the question of which source of netrin1 promotes ventrally directed axon growth. Here, we use genetic approaches in mice to selectively remove netrin from different regions of the spinal cord. Our analyses show that the FP is not the source of netrin1 directing axons to the ventral midline, while local VZ-supplied netrin1 is required for this step. Furthermore, rather than being present in a gradient, netrin1 protein accumulates on the pial surface adjacent to the path of commissural axon extension. Thus, netrin1 does not act as a long-range secreted chemoattractant for commissural spinal axons but instead promotes ventrally directed axon outgrowth by haptotaxis, i.e., directed growth along an adhesive surface.
原文英語
頁(從 - 到)790-799.e3
期刊Neuron
94
發行號4
DOIs
出版狀態已發佈 - 五月 17 2017

指紋

Axons
Spinal Cord
Chemotactic Factors
Chemotaxis
Growth
Adhesives
Proteins
Axon Guidance
Messenger RNA

ASJC Scopus subject areas

  • Neuroscience(all)

引用此文

Varadarajan, S. G., Kong, J. H., Phan, K. D., Kao, T. J., Panaitof, S. C., Cardin, J., ... Butler, S. J. (2017). Netrin1 Produced by Neural Progenitors, Not Floor Plate Cells, Is Required for Axon Guidance in the Spinal Cord. Neuron, 94(4), 790-799.e3. https://doi.org/10.1016/j.neuron.2017.03.007

Netrin1 Produced by Neural Progenitors, Not Floor Plate Cells, Is Required for Axon Guidance in the Spinal Cord. / Varadarajan, Supraja G.; Kong, Jennifer H.; Phan, Keith D.; Kao, Tzu Jen; Panaitof, S. Carmen; Cardin, Julie; Eltzschig, Holger; Kania, Artur; Novitch, Bennett G.; Butler, Samantha J.

於: Neuron, 卷 94, 編號 4, 17.05.2017, p. 790-799.e3.

研究成果: 雜誌貢獻文章

Varadarajan, SG, Kong, JH, Phan, KD, Kao, TJ, Panaitof, SC, Cardin, J, Eltzschig, H, Kania, A, Novitch, BG & Butler, SJ 2017, 'Netrin1 Produced by Neural Progenitors, Not Floor Plate Cells, Is Required for Axon Guidance in the Spinal Cord', Neuron, 卷 94, 編號 4, 頁 790-799.e3. https://doi.org/10.1016/j.neuron.2017.03.007
Varadarajan, Supraja G. ; Kong, Jennifer H. ; Phan, Keith D. ; Kao, Tzu Jen ; Panaitof, S. Carmen ; Cardin, Julie ; Eltzschig, Holger ; Kania, Artur ; Novitch, Bennett G. ; Butler, Samantha J. / Netrin1 Produced by Neural Progenitors, Not Floor Plate Cells, Is Required for Axon Guidance in the Spinal Cord. 於: Neuron. 2017 ; 卷 94, 編號 4. 頁 790-799.e3.
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