Neomycin, spermine and hexaamminecobalt(III) share common structural motifs in converting B- to A-DNA

Howard Robinson, Andrew H.J. Wang

研究成果: 雜誌貢獻文章同行評審

118 引文 斯高帕斯(Scopus)

摘要

The (dG)(n)·(dC)(n)-containing 34mer DNA duplex [d(A2G15C15T2)]2 can be effectively converted from the B-DNA to the A-DNA conformation by neomycin, spermine and Co(NH3)63+. Conversion is demonstrated by a characteristic red shift in the circular dichroism spectra and dramatic NMR spectral changes in chemical shifts. Additional support comes from the substantially stronger CH6/GH8-H3' NOE intensities of the ligand-DNA complexes than those from the native DNA duplex. Such changes are consistent with a deoxyribose pucker transition from the predominate C2'-endo (S-type) to the C3'-endo (N-type). The changes for all three ligand-DNA complexes are identical, suggesting that those three complex cations share common structural motifs for the B- to A-DNA conversion. The A-DNA structure of the 4:1 complex of Co(NH3)63+/d(ACCCGCGGGT) has been analyzed by NOE-restrained refinement. The structural basis of the transition may be related to the closeness of the two negatively charged sugar-phosphate backbones along the major groove in A-DNA, which can be effectively neutralized by the multivalent positively charged amine functions of these ligands. In addition, ligands like spermine or Co(NH3)63+ can adhere to guanine bases in the deep major groove of the double helix, as is evident from the significant direct NOE cross-peaks from the protons of Co(NH3)63+ to GH8, GH1 (imino) and CH4 (amino) protons. Our results point to future directions in preparing more potent derivatives of Co(NH3)63+ for RNA binding or the induction of A-DNA.

原文英語
頁(從 - 到)676-682
頁數7
期刊Nucleic Acids Research
24
發行號4
DOIs
出版狀態已發佈 - 1996
對外發佈

ASJC Scopus subject areas

  • 遺傳學

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