Nascent chromatin capture proteomics determines chromatin dynamics during DNA replication and identifies unknown fork components

Constance Alabert, Jimi-Carlo Bukowski-Wills, Sung-Bau Lee, Georg Kustatscher, Kyosuke Nakamura, Flavia de Lima Alves, Patrice Menard, Jakob Mejlvang, Juri Rappsilber, Anja Groth

研究成果: 雜誌貢獻文章同行評審

217 引文 斯高帕斯(Scopus)

摘要

To maintain genome function and stability, DNA sequence and its organization into chromatin must be duplicated during cell division. Understanding how entire chromosomes are copied remains a major challenge. Here, we use nascent chromatin capture (NCC) to profile chromatin proteome dynamics during replication in human cells. NCC relies on biotin-dUTP labelling of replicating DNA, affinity purification and quantitative proteomics. Comparing nascent chromatin with mature post-replicative chromatin, we provide association dynamics for 3,995 proteins. The replication machinery and 485 chromatin factors such as CAF-1, DNMT1 and SUV39h1 are enriched in nascent chromatin, whereas 170 factors including histone H1, DNMT3, MBD1-3 and PRC1 show delayed association. This correlates with H4K5K12diAc removal and H3K9me1 accumulation, whereas H3K27me3 and H3K9me3 remain unchanged. Finally, we combine NCC enrichment with experimentally derived chromatin probabilities to predict a function in nascent chromatin for 93 uncharacterized proteins, and identify FAM111A as a replication factor required for PCNA loading. Together, this provides an extensive resource to understand genome and epigenome maintenance.
原文英語
頁(從 - 到)281-93
頁數13
期刊Nature Cell Biology
16
發行號3
DOIs
出版狀態已發佈 - 2月 23 2014
對外發佈

指紋

深入研究「Nascent chromatin capture proteomics determines chromatin dynamics during DNA replication and identifies unknown fork components」主題。共同形成了獨特的指紋。

引用此