Nanostructured titanium dioxide layer combined with reactive functional groups as a promising biofunctional surface for biomedical applications

Chi Ming Wu, Chung Ming Liu, Keng Liang Ou, Hsi Jen Chiang, Erwan Sugiatno, Chia Hung Wu, Hsiu Ju Yen, Hsin Hua Chou

研究成果: 雜誌貢獻文章

1 引文 (Scopus)

摘要

In the present study, low-temperature plasma combined with allylamine polymerization approach was used to coat amino-groups on oxide layer of plasma-oxidized biomedical titanium (Ti) for protein immobilization. Scanning electron microscopy, X-ray diffractometer, transmission electron microscopy, X-ray photoemission spectroscopy, secondary ion mass spectrometer and 2, 4, 6-trinitrobenzenesulfonic acid assay were utilized to investigate the surface and microstructural properties of the plasma-oxidized, plasma-polymerized and protein-immobilized samples. Analytical results indicated that the presence of a nanostructured rutile-TiO 2 thin layer could be found on the plasma-oxidized samples. The thickness of nanostructured rutile-TiO 2 layer increased with increasing plasma treatment power and period. As the plasma-oxidized samples underwent plasma polymerization with allylamine, amino-groups (NH 2 ) were uniformly coated on nanostructured rutile-TiO 2 layer. It was also found that Ti surface with thick oxide layer exhibited higher amounts of amino-groups deposition. After protein immobilization, the plasma-polymerized samples presented a formation of uniform streak-like immobilized protein clusters. Therefore, biomedical Ti with nanostructured rutile-TiO 2 layer is a promising biomaterial that can be applied to cross-link with other biomolecules for promoting the bone healing and regeneration.
原文英語
期刊Ceramics International
DOIs
出版狀態已發佈 - 一月 1 2019

指紋

Titanium dioxide
Functional groups
Plasmas
Titanium
Allylamine
Immobilized Proteins
Proteins
Oxides
Trinitrobenzenesulfonic Acid
Plasma polymerization
titanium dioxide
Diffractometers
Biocompatible Materials
Biomolecules
Mass spectrometers
Photoelectron spectroscopy
X ray spectroscopy
Biomaterials
Assays
Bone

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Process Chemistry and Technology
  • Surfaces, Coatings and Films
  • Materials Chemistry

引用此文

Nanostructured titanium dioxide layer combined with reactive functional groups as a promising biofunctional surface for biomedical applications. / Wu, Chi Ming; Liu, Chung Ming; Ou, Keng Liang; Chiang, Hsi Jen; Sugiatno, Erwan; Wu, Chia Hung; Yen, Hsiu Ju; Chou, Hsin Hua.

於: Ceramics International, 01.01.2019.

研究成果: 雜誌貢獻文章

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title = "Nanostructured titanium dioxide layer combined with reactive functional groups as a promising biofunctional surface for biomedical applications",
abstract = "In the present study, low-temperature plasma combined with allylamine polymerization approach was used to coat amino-groups on oxide layer of plasma-oxidized biomedical titanium (Ti) for protein immobilization. Scanning electron microscopy, X-ray diffractometer, transmission electron microscopy, X-ray photoemission spectroscopy, secondary ion mass spectrometer and 2, 4, 6-trinitrobenzenesulfonic acid assay were utilized to investigate the surface and microstructural properties of the plasma-oxidized, plasma-polymerized and protein-immobilized samples. Analytical results indicated that the presence of a nanostructured rutile-TiO 2 thin layer could be found on the plasma-oxidized samples. The thickness of nanostructured rutile-TiO 2 layer increased with increasing plasma treatment power and period. As the plasma-oxidized samples underwent plasma polymerization with allylamine, amino-groups (NH 2 ) were uniformly coated on nanostructured rutile-TiO 2 layer. It was also found that Ti surface with thick oxide layer exhibited higher amounts of amino-groups deposition. After protein immobilization, the plasma-polymerized samples presented a formation of uniform streak-like immobilized protein clusters. Therefore, biomedical Ti with nanostructured rutile-TiO 2 layer is a promising biomaterial that can be applied to cross-link with other biomolecules for promoting the bone healing and regeneration.",
keywords = "Allylamine polymerization, Low-temperature plasma, Oxidation, Protein immobilization",
author = "Wu, {Chi Ming} and Liu, {Chung Ming} and Ou, {Keng Liang} and Chiang, {Hsi Jen} and Erwan Sugiatno and Wu, {Chia Hung} and Yen, {Hsiu Ju} and Chou, {Hsin Hua}",
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AU - Wu, Chi Ming

AU - Liu, Chung Ming

AU - Ou, Keng Liang

AU - Chiang, Hsi Jen

AU - Sugiatno, Erwan

AU - Wu, Chia Hung

AU - Yen, Hsiu Ju

AU - Chou, Hsin Hua

PY - 2019/1/1

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N2 - In the present study, low-temperature plasma combined with allylamine polymerization approach was used to coat amino-groups on oxide layer of plasma-oxidized biomedical titanium (Ti) for protein immobilization. Scanning electron microscopy, X-ray diffractometer, transmission electron microscopy, X-ray photoemission spectroscopy, secondary ion mass spectrometer and 2, 4, 6-trinitrobenzenesulfonic acid assay were utilized to investigate the surface and microstructural properties of the plasma-oxidized, plasma-polymerized and protein-immobilized samples. Analytical results indicated that the presence of a nanostructured rutile-TiO 2 thin layer could be found on the plasma-oxidized samples. The thickness of nanostructured rutile-TiO 2 layer increased with increasing plasma treatment power and period. As the plasma-oxidized samples underwent plasma polymerization with allylamine, amino-groups (NH 2 ) were uniformly coated on nanostructured rutile-TiO 2 layer. It was also found that Ti surface with thick oxide layer exhibited higher amounts of amino-groups deposition. After protein immobilization, the plasma-polymerized samples presented a formation of uniform streak-like immobilized protein clusters. Therefore, biomedical Ti with nanostructured rutile-TiO 2 layer is a promising biomaterial that can be applied to cross-link with other biomolecules for promoting the bone healing and regeneration.

AB - In the present study, low-temperature plasma combined with allylamine polymerization approach was used to coat amino-groups on oxide layer of plasma-oxidized biomedical titanium (Ti) for protein immobilization. Scanning electron microscopy, X-ray diffractometer, transmission electron microscopy, X-ray photoemission spectroscopy, secondary ion mass spectrometer and 2, 4, 6-trinitrobenzenesulfonic acid assay were utilized to investigate the surface and microstructural properties of the plasma-oxidized, plasma-polymerized and protein-immobilized samples. Analytical results indicated that the presence of a nanostructured rutile-TiO 2 thin layer could be found on the plasma-oxidized samples. The thickness of nanostructured rutile-TiO 2 layer increased with increasing plasma treatment power and period. As the plasma-oxidized samples underwent plasma polymerization with allylamine, amino-groups (NH 2 ) were uniformly coated on nanostructured rutile-TiO 2 layer. It was also found that Ti surface with thick oxide layer exhibited higher amounts of amino-groups deposition. After protein immobilization, the plasma-polymerized samples presented a formation of uniform streak-like immobilized protein clusters. Therefore, biomedical Ti with nanostructured rutile-TiO 2 layer is a promising biomaterial that can be applied to cross-link with other biomolecules for promoting the bone healing and regeneration.

KW - Allylamine polymerization

KW - Low-temperature plasma

KW - Oxidation

KW - Protein immobilization

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