Nanopolymeric micelle effect on the transdermal permeability, the bioavailability and gene expression of plasmid

Yaw Chong Tong, Ting Yu Yu, Shwu Fen Chang, Jiahorng Liaw

研究成果: 雜誌貢獻文章同行評審

8 引文 斯高帕斯(Scopus)

摘要

This study attempts to investigate the transdermal permeability, the bioavailability and gene expression of plasmid formulated with nonionic poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) (PEO-PPO-PEO) polymeric micelles (PM). Dynamic light scattering (DLS) and atomic force microscopy (AFM) were used to analyze the PM formulated pCMV-Lac Z (P/PM) containing the gene for β-galactosidase (β-Gal) driven by cytomegalovirus early promoter. Franz diffusion cell was used for in vitro transdermal permeability analysis. Real-time PCR was used to quantify the permeated plasmid in vitro and in vivo. β-Gal activity assay was performed to evaluate transgene expression in vivo. The size of P/PM was ∼50 nm with round shape. PM significantly enhanced the in vitro transdermal permeability of plasmid in a direction- and temperature-dependent manner. Following transdermal application of P/PM, higher area under the curve (AUC P/PM: 98.34 hng/mL) and longer half-life of plasmid were detected compared with that of plasmid alone (AUC P: 10.12 hng/mL). Additionally, the β-Gal activity was significantly increased in skin, stomach, brain and spinal cord at both 48 and 72 h after P/PM application and in testis and spleen at 72 h postapplication. In conclusion, PM formulation enhanced the permeation of plasmid through skin into blood circulation, increasing its absorption and the transgene expression in various tissues.

原文英語
頁(從 - 到)111-120
頁數10
期刊Molecular Pharmaceutics
9
發行號1
DOIs
出版狀態已發佈 - 一月 1 2012

ASJC Scopus subject areas

  • 藥學科學
  • 分子醫學
  • 藥物發現

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