Myeloperoxidase serves as a marker of oxidative stress during single haemodialysis session using two different biocompatible dialysis membranes

Chia Chao Wu, Jin Shuen Chen, Wen Mein Wu, Tung Nan Liao, Pauling Chu, Shih Hua Lin, Chien Huei Chuang, Yuh Feng Lin

研究成果: 雜誌貢獻文章

59 引文 (Scopus)

摘要

Background. There is increased oxidative stress in patients undergoing haemodialysis (HD); however, little is known of how different dialysis membranes contribute to the oxidative stress induced by the dialysis procedure per se. We therefore studied the influence of two different dialysis membranes on oxidative stress during HD. Methods. Eight patients undergoing HD three times per week were enrolled in this cross-controlled study. Patients sequentially received HD using polysulphone (PS) and regenerated cellulose (RC) dialysis membranes for 1 week each. Blood samples were collected in the last section of each hollow fibre 0, 15, 120 and 240 min after starting HD. We determined superoxide anion production derived from neutrophils, superoxide dismutase (SOD) and glutathione peroxidase (GPx) derived from washed red cells, plasma myeloperoxidase (MPO), plasma thiobarbituric acid-reactive substances (TBARS), plasma advanced oxidation protein products (AOPP) and serum 8-hydroxy-2′-deoxyguanosine (8-OHdG). Results. Leukocyte numbers, including neutrophils, lymphocytes and monocytes, decreased significantly after 15 min of dialysis, more so with RC than with PS membrane. For both membranes, superoxide anion production transiently increased during the first 15 min whereas the post-dialysis production was decreased. Plasma MPO levels persistently increased during dialysis with the two membranes. Moreover, the increase was more marked with RC than with PS membrane. AOPP and 8-OHdG levels increased progressively when using RC membranes. There were no significant differences in SOD, GPx, TBARS, AOPP and 8-OHdG levels between the two membranes. Conclusions. The biocompatibility of the dialyser affects oxidative stress production during a single dialysis session. The measurement of MPO may serve as a reliable marker of the degree of oxidative stress induced using dialysis membranes of different biocompatibilities.

原文英語
頁(從 - 到)1134-1139
頁數6
期刊Nephrology Dialysis Transplantation
20
DOIs
出版狀態已發佈 - 六月 2005
對外發佈Yes

指紋

Peroxidase
Renal Dialysis
Dialysis
Oxidative Stress
Membranes
Advanced Oxidation Protein Products
Cellulose
Thiobarbituric Acid Reactive Substances
Glutathione Peroxidase
Superoxides
Superoxide Dismutase
Neutrophils
Plasma Cells
Leukocyte Count
Monocytes
Lymphocytes
Serum

ASJC Scopus subject areas

  • Nephrology
  • Transplantation

引用此文

Myeloperoxidase serves as a marker of oxidative stress during single haemodialysis session using two different biocompatible dialysis membranes. / Wu, Chia Chao; Chen, Jin Shuen; Wu, Wen Mein; Liao, Tung Nan; Chu, Pauling; Lin, Shih Hua; Chuang, Chien Huei; Lin, Yuh Feng.

於: Nephrology Dialysis Transplantation, 卷 20, 06.2005, p. 1134-1139.

研究成果: 雜誌貢獻文章

Wu, Chia Chao ; Chen, Jin Shuen ; Wu, Wen Mein ; Liao, Tung Nan ; Chu, Pauling ; Lin, Shih Hua ; Chuang, Chien Huei ; Lin, Yuh Feng. / Myeloperoxidase serves as a marker of oxidative stress during single haemodialysis session using two different biocompatible dialysis membranes. 於: Nephrology Dialysis Transplantation. 2005 ; 卷 20. 頁 1134-1139.
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abstract = "Background. There is increased oxidative stress in patients undergoing haemodialysis (HD); however, little is known of how different dialysis membranes contribute to the oxidative stress induced by the dialysis procedure per se. We therefore studied the influence of two different dialysis membranes on oxidative stress during HD. Methods. Eight patients undergoing HD three times per week were enrolled in this cross-controlled study. Patients sequentially received HD using polysulphone (PS) and regenerated cellulose (RC) dialysis membranes for 1 week each. Blood samples were collected in the last section of each hollow fibre 0, 15, 120 and 240 min after starting HD. We determined superoxide anion production derived from neutrophils, superoxide dismutase (SOD) and glutathione peroxidase (GPx) derived from washed red cells, plasma myeloperoxidase (MPO), plasma thiobarbituric acid-reactive substances (TBARS), plasma advanced oxidation protein products (AOPP) and serum 8-hydroxy-2′-deoxyguanosine (8-OHdG). Results. Leukocyte numbers, including neutrophils, lymphocytes and monocytes, decreased significantly after 15 min of dialysis, more so with RC than with PS membrane. For both membranes, superoxide anion production transiently increased during the first 15 min whereas the post-dialysis production was decreased. Plasma MPO levels persistently increased during dialysis with the two membranes. Moreover, the increase was more marked with RC than with PS membrane. AOPP and 8-OHdG levels increased progressively when using RC membranes. There were no significant differences in SOD, GPx, TBARS, AOPP and 8-OHdG levels between the two membranes. Conclusions. The biocompatibility of the dialyser affects oxidative stress production during a single dialysis session. The measurement of MPO may serve as a reliable marker of the degree of oxidative stress induced using dialysis membranes of different biocompatibilities.",
keywords = "Antioxidants, Biocompatibility, Haemodialysis, Myeloperoxidase, Oxidative stress",
author = "Wu, {Chia Chao} and Chen, {Jin Shuen} and Wu, {Wen Mein} and Liao, {Tung Nan} and Pauling Chu and Lin, {Shih Hua} and Chuang, {Chien Huei} and Lin, {Yuh Feng}",
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pages = "1134--1139",
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TY - JOUR

T1 - Myeloperoxidase serves as a marker of oxidative stress during single haemodialysis session using two different biocompatible dialysis membranes

AU - Wu, Chia Chao

AU - Chen, Jin Shuen

AU - Wu, Wen Mein

AU - Liao, Tung Nan

AU - Chu, Pauling

AU - Lin, Shih Hua

AU - Chuang, Chien Huei

AU - Lin, Yuh Feng

PY - 2005/6

Y1 - 2005/6

N2 - Background. There is increased oxidative stress in patients undergoing haemodialysis (HD); however, little is known of how different dialysis membranes contribute to the oxidative stress induced by the dialysis procedure per se. We therefore studied the influence of two different dialysis membranes on oxidative stress during HD. Methods. Eight patients undergoing HD three times per week were enrolled in this cross-controlled study. Patients sequentially received HD using polysulphone (PS) and regenerated cellulose (RC) dialysis membranes for 1 week each. Blood samples were collected in the last section of each hollow fibre 0, 15, 120 and 240 min after starting HD. We determined superoxide anion production derived from neutrophils, superoxide dismutase (SOD) and glutathione peroxidase (GPx) derived from washed red cells, plasma myeloperoxidase (MPO), plasma thiobarbituric acid-reactive substances (TBARS), plasma advanced oxidation protein products (AOPP) and serum 8-hydroxy-2′-deoxyguanosine (8-OHdG). Results. Leukocyte numbers, including neutrophils, lymphocytes and monocytes, decreased significantly after 15 min of dialysis, more so with RC than with PS membrane. For both membranes, superoxide anion production transiently increased during the first 15 min whereas the post-dialysis production was decreased. Plasma MPO levels persistently increased during dialysis with the two membranes. Moreover, the increase was more marked with RC than with PS membrane. AOPP and 8-OHdG levels increased progressively when using RC membranes. There were no significant differences in SOD, GPx, TBARS, AOPP and 8-OHdG levels between the two membranes. Conclusions. The biocompatibility of the dialyser affects oxidative stress production during a single dialysis session. The measurement of MPO may serve as a reliable marker of the degree of oxidative stress induced using dialysis membranes of different biocompatibilities.

AB - Background. There is increased oxidative stress in patients undergoing haemodialysis (HD); however, little is known of how different dialysis membranes contribute to the oxidative stress induced by the dialysis procedure per se. We therefore studied the influence of two different dialysis membranes on oxidative stress during HD. Methods. Eight patients undergoing HD three times per week were enrolled in this cross-controlled study. Patients sequentially received HD using polysulphone (PS) and regenerated cellulose (RC) dialysis membranes for 1 week each. Blood samples were collected in the last section of each hollow fibre 0, 15, 120 and 240 min after starting HD. We determined superoxide anion production derived from neutrophils, superoxide dismutase (SOD) and glutathione peroxidase (GPx) derived from washed red cells, plasma myeloperoxidase (MPO), plasma thiobarbituric acid-reactive substances (TBARS), plasma advanced oxidation protein products (AOPP) and serum 8-hydroxy-2′-deoxyguanosine (8-OHdG). Results. Leukocyte numbers, including neutrophils, lymphocytes and monocytes, decreased significantly after 15 min of dialysis, more so with RC than with PS membrane. For both membranes, superoxide anion production transiently increased during the first 15 min whereas the post-dialysis production was decreased. Plasma MPO levels persistently increased during dialysis with the two membranes. Moreover, the increase was more marked with RC than with PS membrane. AOPP and 8-OHdG levels increased progressively when using RC membranes. There were no significant differences in SOD, GPx, TBARS, AOPP and 8-OHdG levels between the two membranes. Conclusions. The biocompatibility of the dialyser affects oxidative stress production during a single dialysis session. The measurement of MPO may serve as a reliable marker of the degree of oxidative stress induced using dialysis membranes of different biocompatibilities.

KW - Antioxidants

KW - Biocompatibility

KW - Haemodialysis

KW - Myeloperoxidase

KW - Oxidative stress

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U2 - 10.1093/ndt/gfh764

DO - 10.1093/ndt/gfh764

M3 - Article

C2 - 15814542

AN - SCOPUS:20844455941

VL - 20

SP - 1134

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JO - Nephrology Dialysis Transplantation

JF - Nephrology Dialysis Transplantation

SN - 0931-0509

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