Mycelia from Antrodia camphorata in submerged culture induce apoptosis of human hepatoma HepG2 cells possibly through regulation of fas pathway

Tuzz Ying Song, Shih Lan Hsu, Chi T. Yeh, Gow Chin Yen

研究成果: 雜誌貢獻文章

50 引文 (Scopus)

摘要

The objective of this study was to investigate the antiproliferative effect and the mechanism of the methanol extracts of mycelia (MEM) form Antrodia camphorata in submerged culture toward HepG2 cells. The results showed that MEM-induced cell apoptosis involved up-regulation of Fas and down-regulation of Bcl-2, DR3, DR4, TNFRI, and TNFRII in HepG2 cells, while no changes on the levels of Bax, Bid, Bad, and Bak protein were observed. On the basis of these results, the involvement of the Fas/Fas ligand (FasL) death-receptor pathway, in MEM-induced apoptosis in HepG2 cells, was investigated. The apoptosis inducing activity was significantly enhanced by a Fas activator and inhibited by a Fas antagonist. To know about the effect of MEM on the activation of the apoptotic pathway, the adenovirus transfected with Bcl-2 was infected on HepG2 cells. The data showed that the percentage of apoptotic cells induced by MEM in Bcl-2-infected HepG2 (Bcl-2 overexpression) was not significantly different from that of uninfected HepG2. These results demonstrate that MEM induces HepG2 apoptosis through inhibition of cell growth and up-regulation of Fas/FasL to activate the pathway of caspase-3 and -8 cascades.
原文英語
頁(從 - 到)5559-5564
頁數6
期刊Journal of Agricultural and Food Chemistry
53
發行號14
DOIs
出版狀態已發佈 - 七月 13 2005
對外發佈Yes

指紋

Antrodia
Taiwanofungus camphoratus
Mycelium
Hep G2 Cells
hepatoma
mycelium
Methanol
Hepatocellular Carcinoma
methanol
apoptosis
Apoptosis
extracts
Fas Ligand Protein
cells
BH3 Interacting Domain Death Agonist Protein
bcl-2 Homologous Antagonist-Killer Protein
bcl-Associated Death Protein
Up-Regulation
bcl-2-Associated X Protein
Death Domain Receptors

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Chemistry (miscellaneous)

引用此文

Mycelia from Antrodia camphorata in submerged culture induce apoptosis of human hepatoma HepG2 cells possibly through regulation of fas pathway. / Song, Tuzz Ying; Hsu, Shih Lan; Yeh, Chi T.; Yen, Gow Chin.

於: Journal of Agricultural and Food Chemistry, 卷 53, 編號 14, 13.07.2005, p. 5559-5564.

研究成果: 雜誌貢獻文章

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abstract = "The objective of this study was to investigate the antiproliferative effect and the mechanism of the methanol extracts of mycelia (MEM) form Antrodia camphorata in submerged culture toward HepG2 cells. The results showed that MEM-induced cell apoptosis involved up-regulation of Fas and down-regulation of Bcl-2, DR3, DR4, TNFRI, and TNFRII in HepG2 cells, while no changes on the levels of Bax, Bid, Bad, and Bak protein were observed. On the basis of these results, the involvement of the Fas/Fas ligand (FasL) death-receptor pathway, in MEM-induced apoptosis in HepG2 cells, was investigated. The apoptosis inducing activity was significantly enhanced by a Fas activator and inhibited by a Fas antagonist. To know about the effect of MEM on the activation of the apoptotic pathway, the adenovirus transfected with Bcl-2 was infected on HepG2 cells. The data showed that the percentage of apoptotic cells induced by MEM in Bcl-2-infected HepG2 (Bcl-2 overexpression) was not significantly different from that of uninfected HepG2. These results demonstrate that MEM induces HepG2 apoptosis through inhibition of cell growth and up-regulation of Fas/FasL to activate the pathway of caspase-3 and -8 cascades.",
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