Murine mast cells secrete and respond to interleukin-33

Hui Ying Tung, Beverly Plunkett, Shau Ku Huang, Yufeng Zhou

研究成果: 雜誌貢獻文章

34 引文 斯高帕斯(Scopus)

摘要

Interleukin-33 (IL-33) appears to play a crucial role in the expression of allergic diseases, but its cellular source and regulatory mechanisms remain to be fully elucidated. Mast cells, one of the major effecter cell populations in mediating allergy, express high levels of IL-33 receptor, ST2, and have been shown to express IL-33 transcripts. In this study, we aimed to examine the secretion of IL-33 in mast cells and their response to IL-33. We have successfully detected secreted IL-33 from cell supernatants through a modified enzyme-linked immunosorbent assay (ELISA) technique - cell-based ELISA. Activation of bone marrow-derived cultured mast cells (BMMCs) by crosslinkage of an antigen [ovalbumin (OVA)] and OVA-specific IgE mAbs significantly induced the expression of IL-33 transcripts, cytosolic and secreted proteins. In addition, the Toll-like receptor (TLR) 2 and TLR-9 ligands could trigger IL-33 mRNA expression. Exposure of BMMCs to IL-33 significantly increased the levels of IL-13 and IL-6 expression, concomitant with enhanced activation of mitogen-activated protein kinase (MAPKs) (ERK, p38, and JNK) and nuclear factor-kappa B. These results suggest that mouse BMMCs are capable of producing and serving as endogenous sources of IL-33, and that IL-33 plays an important role in regulating mast cell functions.

原文英語
頁(從 - 到)141-147
頁數7
期刊Journal of Interferon and Cytokine Research
34
發行號3
DOIs
出版狀態已發佈 - 三月 1 2014
對外發佈Yes

    指紋

ASJC Scopus subject areas

  • Immunology
  • Cell Biology
  • Virology

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